BLIMP1/PRDM1 Antibody (646702) [Allophycocyanin]

FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." > FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." title="Human peripheral blood mononuclear cells (PBMCs) gated on CD19⁺CD3^-cells were stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." />

Human peripheral blood mononuclear cells (PBMCs) gated on CD19⁺CD3^-cells were stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # <a class=

IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." > IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." title="U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." />

U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A, filled histogram) or isotype control antibody (Catalog # <a class=

• Reactivity: Hu
• Applications: Flow
• Clone: 646702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

BLIMP1/PRDM1 Antibody (646702) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human BLIMP1/PRDM1
  Lys667-Cys789
  Accession # O75626
• Specificity: Detects human BLIMP1/PRDM1 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BLIMP1/PRDM1 Antibody (646702) [Allophycocyanin]

• Beta-interferon gene positive regulatory domain I-binding factor
• beta-interferon gene positive-regulatory domain I binding factor
• BLIMP1
• BLIMP-1
• BLIMP1MGC118925
• blmp-1
• B-lymphocyte-induced maturation protein 1
• MGC118922
• MGC118923
• Positive regulatory domain I-binding factor 1
• PR domain containing 1, with ZNF domain
• PR domain zinc finger protein 1
• PR domain-containing protein 1
• PRDI-BF1MGC118924
• PRDI-binding factor 1
• PRDI-binding factor-1
• PRDM1
• PR-domain zinc finger protein 1
• ZNFPR1A1

Background

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLIMP1/PRDM1 Antibody (IC36081A)(4)

Publications using IC36081A

• Zhang, H;Okuyama, H;Jain, A;Jadhav, RR;Wu, B;Sturmlechner, I;Morales, J;Ohtsuki, S;Weyand, CM;Goronzy, JJ; PREX1 improves homeostatic proliferation to maintain a naïve CD4 T cell compartment in older age JCI insight 2024-02-08 [PMID: 38329813] (Flow Cytometry, Human)
• Tuan Anh Le, Van Trung Chu, Andreia C. Lino, Eva Schrezenmeier, Christopher Kressler, Dania Hamo et al. Efficient CRISPR-Cas9-mediated mutagenesis in primary human B cells for identifying plasma cell regulators Molecular Therapy - Nucleic Acids 12/13/2022 [PMID: 36514352]
• Arshi Khanam, Natarajan Ayithan, Lydia Tang, Bhawna Poonia, Shyam Kottilil IL-21–Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B Frontiers in Immunology 1/28/2021 [PMID: 33584666]
• JA Bibby, HA Purvis, T Hayday, A Chandra, K Okkenhaug, S Rosenzweig, I Aksentijev, M Wood, HJ Lachmann, C Kemper, AP Cope, E Perucha Cholesterol metabolism drives regulatory B cell IL-10 through provision of geranylgeranyl pyrophosphate Nat Commun, 2020-07-08;11(1):3412. 2020-07-08 [PMID: 32641742] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: PRDM1
• Uniprot:
  • O75626()