BIRC6 Antibody - BSA Free

Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).

Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BIRC6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BIRC6 Antibody - BSA Free (NB300-264) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-BIRC6 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide derived from a sequence between residue 4775 and the C-terminus (residue 4829) of human Baculoviral IAP repeat-containing protein 6 using the numbering given in Swiss-Prot entry Q9NR09 (GeneID 57448).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIRC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:33815677) Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BIRC6 Antibody - BSA Free

• APOLLON
• baculoviral IAP repeat containing 6
• baculoviral IAP repeat-containing 6
• baculoviral IAP repeat-containing protein 6
• BIRC6
• BRUCE
• EC 2.3.2.27
• FLJ13786
• KIAA1289
• KIAA1289FLJ13726
• Ubiquitin-conjugating BIR domain enzyme apollon
• ubiquitin-conjugating BIR-domain enzyme apollon

Background

This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BIRC6 Antibody (NB300-264)(3)

Publications using NB300-264

• Vilfranc CL Elucidating the Role of BRUCE in Chronic Liver Disease Pathogenesis Thesis 2021-01-01
• Vilfranc CL, Che LX, Patra KC et al. BIR repeat-containing ubiquitin conjugating enzyme (BRUCE) regulation of beta-catenin signaling in the progression of drug-induced hepatic fibrosis and carcinogenesis World journal of hepatology 2021-03-27 [PMID: 33815677] (ICC/IF, Mouse)
• Aharinejad S, Andrukhova O, Lucas T et al. Programmed cell death in idiopathic dilated cardiomyopathy is mediated by suppression of the apoptosis inhibitor Apollon. Ann Thorac Surg 2008-07-01 [PMID: 18573408]

Bioinformatics

• Gene Symbol: BIRC6
• Entrez:
  • 57448(Human)
• Uniprot:
  • Q9NR09(Human)