BID [p Ser78] Antibody - BSA Free

Western Blot: BID [p Ser78] Antibody [NB500-260] - Detection of Mouse Phospho-BID (S78) after Treatment with EPE. Whole cell lysate (50 and 80 ug for WB; 1 mg for IP, 20% of IP loaded) from NIH3T3 cells that were treated with etoposide (100 mcM; 3 hours) or mock treated. Antibody used at 1 ug/ml.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

BID [p Ser78] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BID [p Ser78] Antibody - BSA Free (NB500-260) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to phosphorylated Serine 78 and surrounding residues mouse BH3 interacting domain death agonist using the numbering given in entry NP_031570.2 (GeneID 12122).
• Modification: p Ser78
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BID
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BID [p Ser78] Antibody - BSA Free

• apoptic death agonist
• BH3 interacting domain death agonist
• BH3-interacting domain death agonist
• BID isoform ES(1b)
• BID isoform L(2)
• BID isoform Si6
• BID
• desmocollin type 4
• FP497
• Human BID coding sequence
• MGC15319
• MGC42355
• p22 BID
• tbid

Background

Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for BID Antibody (NB500-260). (Showing 1 - 2 of 2 FAQ).

1. I have a question regarding one of your antibodies. It is for catalog #NB500-260, Rabbit anti-mouse BID (pSer78). Do you have any Western Blot images and/or links to published data?
   • Thank you for taking an interest in our Bid Antibody. Unfortunately we do not have any references but we do have a western blot image. I would like to reassure you with our full money back guarantee if you find that the antibody does not work.
2. Hi, I'm interested in some information about your rabbit anti-BID phospho Ser78 antibody (cat# NB500-260). Is the band expected at ~22kDa and is it a single band that we should expect? for both mouse and human?
   • BID is expressed as a number of different isoforms and is subject to phosphorylation, therefore you should expect to see multiple bands in your western blot around 22 kDa, but this will depend on the specific expression pattern in your samples. This is true for both the mouse and human proteins.

Bioinformatics

• Gene Symbol: BID
• Entrez:
  • 12122(Mouse)
• Uniprot:
  • P70444()