Novus Biologicals products are now on bio-techne.com

beta Tubulin Antibody

Images

 
Western Blot: beta Tubulin Antibody [NB100-56459] - Analysis of beta-Tubulin in A: human brain, B: mouse brain and C: rat brain tissue lysates using the this antibody at 25 ug/mL.
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NB100-56459] - Human kidney tubules stained with beta-Tubulin antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 ...read more
Simple Western: beta Tubulin Antibody [NB100-56459] - Image shows a specific band for beta Tubulin in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, Pm, XpSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

beta Tubulin Antibody Summary

Immunogen
A synthetic peptide corresponding to a portion of amino acids 400-450 of human b-tubulin was used as immunogen for this antibody, GenBank no. gi|27227551|gb|AAN85571.1|.
Specificity
The amino acid sequence used as immunogen has 100% identity in beta I and beta II tubulin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Simple Western 1:50
  • Western Blot 0.1 - 1 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Control
beta II Tubulin A Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB100-56459 in the following applications:

  • WB
    6 publications

Reactivity Notes

Feline reactivity reported in scientific literature (PMID: 25668772).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for beta Tubulin Antibody

  • beta polypeptide
  • beta Tubulin
  • MGC117247
  • OK/SW-cl.56
  • TUBB
  • TUBB5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

Alpha and beta Tubulin are the most common members of the tubulin family of proteins. Dimers of alpha and beta tubulin assemble to form microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-33582
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
DIP100
Species: Hu
Applications: ELISA
H00000730-D01P
Species: Hu, Mu
Applications: WB
MAB1195
Species: All-Multi
Applications: ICC, IHC, ICFlow, Simple Western, WB
NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NLS6168
Species: Hu, Pm, Pm
Applications: Flow (-), Flow, ICC/IF (-), ICC/IF, IHC, IHC-P, IP (-), IP, WB (-), WB
MAB6544
Species: Hu
Applications: CyTOF-ready, Flow, IHC
NBP2-33582
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NBP1-46535
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NBP2-14886
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB600-533
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
NBP2-15581
Species: Dr, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF4309
Species: Hu, Mu
Applications: ICC, IHC, WB
NBP1-84037
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
AF1640
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
H00023268-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
NB100-56459
Species: Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, Pm, Xp
Applications: WB, Simple Western, IHC
Supplier Logo

Publications for beta Tubulin Antibody (NB100-56459)(11)

We have publications tested in 5 confirmed species: Mouse, Rat, Canine, Drosophila, Feline.

We have publications tested in 1 application: WB.


Filter By Application
WB
(6)
All Applications
Filter By Species
Mouse
(2)
Rat
(1)
Canine
(1)
Drosophila
(1)
Feline
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56459 Applications Species
Cheramangalam RN, Anand T, Pandey P et al. Bendless is essential for PINK1-Park mediated Mitofusin degradation under mitochondrial stress caused by loss of LRPPRC PLoS genetics 2023-04-01 [PMID: 37098042] (WB, Drosophila) WB Drosophila
Lima C, Andrade-Barros AI, Bernardo JTG et al. Natterin-Induced Neutrophilia Is Dependent on cGAS/STING Activation via Type I IFN Signaling Pathway International journal of molecular sciences [PMID: 35408954] (WB, Mouse) WB Mouse
Krisko TI, Nicholls HT, Bare CJ et al. Dissociation of Adaptive Thermogenesis from Glucose Homeostasis in Microbiome-Deficient Mice Cell Metab. 2020-02-12 [PMID: 32084379]
R Goulet C, Bernard G, Tremblay S et al. Exosomes Induce Fibroblast Differentiation into Cancer-associated Fibroblasts through TGFb Signaling. Mol. Cancer Res. 2018-04-10 [PMID: 29636362] (WB) WB
Chen M, Arumugam TV, Leanage G et al. Disease-modifying effect of intravenous immunoglobulin in an experimental model of epilepsy Sci Rep. 2017-01-11 [PMID: 28074934] (WB, Mouse) WB Mouse
Williams K, Balsor JL, Beshara S et al. Experience-dependent central vision deficits: Neurobiology and visual acuity Vision Res. 2015-02-07 [PMID: 25668772] (WB, Feline)

Details:
beta-Tubulin antibody used at 1:4000 dilution in WB application for analysing 25ug lysates of the central, peripheral, and monocular regions of visual cortex /V1 section obtained from the brain tissues of 5 week old Feline/Cats which were subjected to 6 hours to 7 days of monocular deprivation (Fig. 1).
WB Feline
Murphy KM, Balsor J, Beshara S et al. A high-throughput semi-automated preparation for filtered synaptoneurosomes J. Neurosci. Methods. 2014-07-02 [PMID: 24997341] (WB, Canine, Rat)

Details:
beta Tubulin antibody used at 1:4000 dilution in WB on whole homogenate and synaptoneurosomes from somatosensory cortex of Long Evans rats and adult cat/feline frontal cortex.
WB Canine, Rat
Saha T. LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy. Autophagy. 2012-11-01 [PMID: 22874552]
Li R, Guan H, Yang K. Neuropeptide Y potentiates beta-adrenergic stimulation of lipolysis in 3T3-L1 adipocytes. Regul Pept. 2012-10-10 [PMID: 22750277]
Kamal A, Sreekanth K, Kumar PP et al. Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines. Eur J Med Chem. 2010-06-01 [PMID: 20171761]

Details:
Products cited for western blot: 1. PARP (IMG-401A): MCF-7 cells treated with numerous compounds, Fig 7. 2. B-Tubulin (IMG-5180A): MCF-7 cells treated with PP-PBDs, Fig 6 and Fig 7. 3. Cytochrome C (IMG-101A): MCF-7 cells treated with numerous compounds,
Show All 11 Publications.

Reviews for beta Tubulin Antibody (NB100-56459) (0)

There are no reviews for beta Tubulin Antibody (NB100-56459). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta Tubulin Antibody (NB100-56459). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for an antibody which is a specific marker for Mouse Bronchial cilia in IHC(p) technique. Preferably not from a mouse host. Can you help?
    • According to the literature I have reviewed on bronchial cilia markers, the most commonly used marker is acetylated alpha tubulin (AcTub). Please see the relevant publications with images attached (Song et al. 2014, Schamberger et al. 2014). We have one antibody to AcTub that is validated for IHC-P and mouse (it is however also raised in mouse, hence mouse on mouse blocking step would be necessary): NB600-567. There are also some reports of using beta tubulin as cilial marker (Davis et al. 2015). For rabbit host antibodies, you may look at our best seller products like NB600-936 and NB100-56459
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our beta Tubulin Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TUBB
Entrez
Uniprot