beta-III Tubulin Antibody (rTUBB3/7405) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human brain stained with beta-III Tubulin antibody (rTUBB3/7405). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: rTUBB3/7405
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

beta-III Tubulin Antibody (rTUBB3/7405) - Azide and BSA Free Summary

• Description: Positive Controls: Human brain or testis. PC3 cells.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa250-450) of human beta-III Tubulin (exact sequence is proprietary)
• Localization: Cytoplasm.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for beta-III Tubulin Antibody (rTUBB3/7405) - Azide and BSA Free

• Beta3-tubulin
• beta-4
• betaIII Tubulin
• beta-III Tubulin
• beta-Tubulin III
• Class III beta-tubulin
• TUBB3
• TUBB4 CFEOM3A
• Tubulin Beta 3
• tubulin beta-3 chain
• Tubulin beta-4 chain
• Tubulin beta-4
• Tubulin beta-III
• tubulin, beta 3
• TUJ1 antigen
• TUJ-1 antigen

Background

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB3