beta I Tubulin Antibody (20B1) - BSA Free

Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Image of beta I Tubulin Antibody (16E2) diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Image of beta I Tubulin Antibody (16E2) diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot: beta I Tubulin Antibody (16E2) [NBP3-26267] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: beta I Tubulin Antibody at 1: 1000 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 50 kDa Observed band size: 50 kDa

Flow Cytometry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Overlay Peak curve showing NIH/3T3 cells stained with beta I Tubulin Antibody (16E2) (red line) at 1:200. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 20B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

beta I Tubulin Antibody (20B1) - BSA Free Summary

• Description: Novus Biologicals Mouse beta I Tubulin Antibody (20B1) - BSA Free (NBP3-26267) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: Recombinant Human beta I Tubulin protein [UniProt Q9H4B7]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol, 0.01M PBS, PH 7.4
• Preservative: 0.03% Proclin 300
• Purity: Affinity purified

Alternate Names for beta I Tubulin Antibody (20B1) - BSA Free

• beta tubulin 1, class VI
• betaI Tubulin
• beta-I Tubulin
• dJ543J19.4
• TUBB1
• tubulin beta-1 chain
• tubulin, beta 1

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB1