beta-Catenin Antibody (1C9) - Azide and BSA Free

Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody (M02), clone 1C9.Lane 1: CTNNB1 transfected lysate(85.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - PC-3 MM2 cells were stained with CTNNB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).

Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi ( Cat # H00001499-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody (M02), clone 1C9 (Cat # H00001499-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of monoclonal antibody to CTNNB1 on HeLa cell. Antibody concentration 10 ug/ml.

Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of protein-protein interactions between GSK3B and CTNNB1. HeLa cells were stained with anti-GSK3B rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: beta-Catenin Antibody (1C9) [H00001499-M02] - Detection limit for recombinant GST tagged CTNNB1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF, KD
• Clone: 1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

beta-Catenin Antibody (1C9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CTNNB1 (AAH58926, 682 a.a. ~ 781 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL
• Marker: Epithelial Cell Marker, Adherens Junction Marker
• Specificity: CTNNB1 (1C9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTNNB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for beta-Catenin Antibody (1C9) - Azide and BSA Free

• bCatenin
• beta 1 (88kD)
• beta-Catenin
• catenin (cadherin-associated protein), beta 1, 88kDa
• catenin beta-1
• CTNNB
• CTNNB1
• DKFZp686D02253
• FLJ25606
• FLJ37923

Background

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Catenin Antibody (H00001499-M02)(1)

Publication using H00001499-M02

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: CTNNB1
• Entrez:
  • 1499(Human)
• OMIM:
  • 114550(Human)
• Uniprot:
  • AAH58926(Human)