beta-Catenin Antibody (196624) [Phycoerythrin]

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta -Catenin PE-conjugated Monoclonal Antibody (Catalog # IC13292P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 196624
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

beta-Catenin Antibody (196624) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human beta -Catenin
  Ala2-Leu781
  Accession # P35222
• Specificity: Detects human, mouse, and rat beta -Catenin in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTNNB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Catenin Antibody (196624) [Phycoerythrin]

• bCatenin
• beta 1 (88kD)
• beta-Catenin
• catenin (cadherin-associated protein), beta 1, 88kDa
• catenin beta-1
• CTNNB
• CTNNB1
• DKFZp686D02253
• FLJ25606
• FLJ37923

Background

beta -Catenin is a 94-96 kDa member of the beta -Catenin family, armadillo-repeat superfamily of intracellular molecules. Human beta -Catenin is 781 amino acids (aa) in length and contains three basic regions; there is a "flexible" N-terminus (aa 1-110) that undergoes phosphorylation, O-linked glycosylation, acetylation and ubiquitination, a central region (aa 14-670) that contains 12 armadillo-like repeats and engages in protein-protein interaction, and a "flexible" C-terminus that also undergoes phosphorylation. beta -Catenin is widely, if not ubiquitously expressed, being found in endothelial cells, NKT cells, cortical neurons, neural stem cells, osteoblasts, fibroblasts, skeletal muscle cells, and Tregs. beta -Catenin is perhaps best known as a facilitator for Wnt signaling. In this context, and in the absence of Wnt signaling, non-nuclear or E-Cadherin-associated beta -Catenin is continually synthesized and degraded via a phosphorylation-dependent program. Upon Wnt engagement of its LPR:Fzd receptor complex, phosphorylation stops, allowing for an increase in it half-life and translocation into the nucleus. Here, it binds to TCF, generating a gene-activating transcriptional complex. Notably, Wnt signaling has been described as being  beta -Catenin and Ca⁺⁺/non-beta -Catenin based. It now appears they work in tandem, with Ca⁺⁺ mobilization facilitating the translocation of beta -Catenin across the nuclear membrane. Human and mouse full-length beta -Catenin shows 99% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Catenin Antibody (IC13292P)(1)

Publication using IC13292P

• AT Alshareeda, E Rakha, A Alghwainem, B Alrfaei, B Alsowayan, A Albugami, AM Alsubayyil, M Abomraee, NK Mohd Zin The effect of human placental chorionic villi derived mesenchymal stem cell on triple-negative breast cancer hallmarks PLoS ONE, 2018-11-20;13(11):e0207593. 2018-11-20 [PMID: 30458011] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CTNNB1
• Uniprot:
  • P35222()