Beclin 1 [p Ser234] Antibody - Azide Free

Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Beclin 1 [p Ser234] Antibody [NBP2-29654] - flag-beclin-1 WT immunoprecipitates expressed in 293T cells showing specific immunolabeling of beclin-1 when phosphorylated at S234 (WT). This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A).

• Reactivity: Hu, Mu, Rt, Mu, Rt, Bv, Ca, Pm, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Beclin 1 [p Ser234] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH. Accession # Q14457. Accession # Q14457
• Modification: p Ser234
• Specificity: Specific for endogenous levels of the ~60 kDa beclin 1 protein phosphorylated at Ser234. Immunolabeling of the beclin 1 band is decreased in the beclin 1 alanine substitution mutant.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Sheep (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BECN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250
• Application Notes: Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antibody has been directly tested for reactivity in Western blots with recombinant HeLa and 293T cells. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, rat, and sheep tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Beclin 1 [p Ser234] Antibody - Azide Free

• ATG6 autophagy related 6 homolog
• ATG6
• beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
• beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
• Beclin 1
• beclin 1, autophagy related
• beclin1
• beclin-1
• BECN1
• Coiled-coil myosin-like BCL2-interacting protein
• GT197
• Protein GT197
• VPS30

Background

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234 (Wang et al., 2012).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beclin 1 Antibody (NBP2-29654)(1)

Publication using NBP2-29654

• Muniz-Feliciano L, Doggett TA, Zhou Z, Ferguson TA. RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye Autophagy 2017-09-21 [PMID: 28933590]

Bioinformatics

• Gene Symbol: BECN1
• Entrez:
  • 8678(Human)
• Uniprot:
  • Q14457(Human)