BCR Antibody [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: Flow, ICC/IF, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Janelia Fluor 669

BCR Antibody [Janelia Fluor® 669] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-19097. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for BCR Antibody [Janelia Fluor® 669]

• ALL
• ALLD22S11
• BCR
• BCR1
• breakpoint cluster region protein
• breakpoint cluster region
• CMLEC 2.7.11.1
• D22S662
• PHL
• PHLFLJ16453
• Renal carcinoma antigen NY-REN-26

Background

The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCR