BCMA/TNFRSF17 Overexpression Lysate (Native)

Images

 
Western Blot: BCMA Overexpression Lysate (Adult Normal) [NBL1-17151] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BCMA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BCMA/TNFRSF17 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208851 and based on accession number NM_001192.
Epitope
C-terminal DDK tag
Specificity
Homo sapiens tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), mRNA.
Gene
TNFRSF17

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for BCMA/TNFRSF17

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BCMA/TNFRSF17 Overexpression Lysate (Native)

  • B cell maturation antigen
  • B-cell maturation protein
  • BCMA
  • BCMAtumor necrosis factor receptor superfamily member 17
  • BCMB-cell maturation factor
  • CD269 antigen
  • CD269
  • TNFRSF17
  • tumor necrosis factor receptor superfamily, member 17

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for BCMA/TNFRSF17 Lysate (NBL1-17151) (0)

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Bioinformatics

Gene Symbol TNFRSF17
Entrez