ANP32B Antibody

Images

 
Western Blot: ANP32B Antibody [NB100-1199] - 0.1 ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ANP32B Antibody [NB100-1199] - LPS/IFN gamma-stimulated neuron-glial co-cultures (Glia/PAL31+ neuron) showing expression of both iNOS and PAL31. Image collected and cropped by ...read more
Immunohistochemistry: ANP32B Antibody [NB100-1199] - (C) Representative micrograph showing colocalization of upregulated PAL31 (green) with microglia/macrophage (ED1 positive cells) (D) Representative micrograph showing ...read more
Western Blot: ANP32B Antibody [NB100-1199] - (0.1 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ANP32B Antibody [NB100-1199] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Genetic Strategies: Knockdown Validated: ANP32B Antibody [NB100-1199] - Western blot analysis of pal31 siRNA-treated C6 showing knockdown of PAL31 expression by PAL31 silencer using 41.5 (lane1), 83 (lane2), and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA, KD
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

ANP32B Antibody Summary

Immunogen
Peptide with sequence C-KRKRETDDEGEDD corresponding to C-Terminus according to NP_006392.1.
Specificity
This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANP32B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3-5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Knockdown Validated
Application Notes
WB: Approx. 26 kDa band observed in human tonsil lysates (calculated MW of 28.8 kDa band according to NP_006392.1). IHC-P: Human breast shows nuclear staining in lobular cells.
Control
ANP32B Overexpression Lysate
Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
ANP32B Peptide (NB100-1199PEP)
Publications
Read Publications using
NB100-1199 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25034417).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ANP32B Antibody

  • acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
  • acidic leucine-rich nuclear phosphoprotein 32 family member B
  • Acidic protein rich in leucines
  • APRILSilver-stainable protein SSP29
  • Pal31
  • PHAPI2SSP29
  • Putative HLA-DR-associated protein I-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ANP32B Antibody (NB100-1199)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ANP32B Antibody (NB100-1199) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANP32B
Entrez
Uniprot