BCMA/TNFRSF17 Antibody [Unconjugated]

U266 human myeloma cell line was stained with Goat Anti-Human/Primate BCMA/TNFRSF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF193, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, B/N, CyTOF-ready, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BCMA/TNFRSF17 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BCMA/TNFRSF17
  Met1-Ala54
  Accession # Q6PE46
• Specificity: Detects human BCMA in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhTACI, and recombinant mouse BCMA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF17
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human BCMA/TNFRSF17 Antibody (Catalog # AF193)
  ELISA Detection: Human BCMA/TNFRSF17 Biotinylated Antibody (Catalog # BAF193)
  Standard: Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCMA/TNFRSF17 Antibody [Unconjugated]

• B cell maturation antigen
• B-cell maturation protein
• BCMA
• BCMAtumor necrosis factor receptor superfamily member 17
• BCMB-cell maturation factor
• CD269 antigen
• CD269
• TNFRSF13A
• TNFRSF17
• tumor necrosis factor receptor superfamily, member 17

Background

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCMA/TNFRSF17 Antibody (AF193)(7)

Publications using AF193

• MC Da Vià, O Dietrich, M Truger, P Arampatzi, J Duell, A Heidemeier, X Zhou, S Danhof, S Kraus, M Chatterjee, M Meggendorf, S Twardziok, ME Goebeler, MS Topp, M Hudecek, S Prommersbe, K Hege, S Kaiser, V Fuhr, N Weinhold, A Rosenwald, F Erhard, C Haferlach, H Einsele, KM Kortüm, AE Saliba, L Rasche Homozygous BCMA gene deletion in response to anti-BCMA CAR T cells in a patient with multiple myeloma Nature Medicine, 2021-02-22;0(0):. 2021-02-22 [PMID: 33619368] (IHC, Human)
• DX Bu, R Singh, EE Choi, M Ruella, S Nunez-Cruz, KG Mansfield, P Bennett, N Barton, Q Wu, J Zhang, Y Wang, L Wei, S Cogan, T Ezell, S Joshi, KJ Latimer, B Granda, WR Tschantz, RM Young, HA Huet, CJ Richardson, MC Milone Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma Oncotarget, 2018-05-25;9(40):25764-25780. 2018-05-25 [PMID: 29899820] (IHC-P, Human)
• Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2015-07-03;30(1):163-72. 2015-07-03 [PMID: 26139429] (Neutralization, Human)
• Sarah A. Laurent, Franziska S. Hoffmann, Peer-Hendrik Kuhn, Qingyu Cheng, Yuanyuan Chu, Marc Schmidt-Supprian et al. gamma -secretase directly sheds the survival receptor BCMA from plasma cells Nature Communications 2015-06-11 [PMID: 26065893]
• Carpenter , Robert O, Evbuomwan , Moses O, Pittaluga , Stefania, Rose , Jeremy J, Raffeld , Mark, Yang , Shicheng, Gress , Ronald E, Hakim , Frances, Kochenderfer , James N B-cell Maturation Antigen is a Promising Target for Adoptive T-cell Therapy of Multiple Myeloma. Clin Cancer Res, 2013-01-23;19(0):2048-60. 2013-01-23 [PMID: 23344265] (IHC-P, Human)
• Daridon C, Devauchelle V, Hutin P, Le Berre R, Martins-Carvalho C, Bendaoud B, Dueymes M, Saraux A, Youinou P, Pers JO Aberrant expression of BAFF by B lymphocytes infiltrating the salivary glands of patients with primary Sjögren&#039;s syndrome. Arthritis Rheum., 2007-04-01;56(4):1134-44. 2007-04-01 [PMID: 17393395] (IHC-Fr, Human)
• Tai YT, Li XF, Breitkreutz I, Song W, Neri P, Catley L, Podar K, Hideshima T, Chauhan D, Raje N, Schlossman R, Richardson P, Munshi NC, Anderson KC Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment. Cancer Res., 2006-07-01;66(13):6675-82. 2006-07-01 [PMID: 16818641] (Flow Cytometry, Human)

Review for BCMA/TNFRSF17 Antibody (AF193) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 01/05/2015

Summary

• Application: ELISA
• Sample Tested: See PMIC 22804669
• Species: Human

Bioinformatics

• Gene Symbol: TNFRSF17
• Uniprot:
  • Q6PE46()