VMAT2 Antibody (OTI9E11)

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - VMAT2 protein level by using anti-VMAT2 (9E11) antibody in the striatum of autopsied human brain.

Immunocytochemistry/Immunofluorescence: VMAT2 Antibody (OTI9E11) [NBP1-47977] - VMAT2 Antibody (9E11) [NBP1-47977] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VMAT2.

Immunohistochemistry-Paraffin: VMAT2 Antibody (9E11) [NBP1-47977] - Staining of paraffin-embedded Human pancreas tissue using anti-VMAT2 mouse monoclonal antibody.

Flow Cytometry: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VMAT2 antibody, and then analyzed by flow cytometry.

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMAT2.

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti SLC18A2 monoclonal antibody.(1:200).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: OTI9E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

VMAT2 Antibody (OTI9E11) Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human SLC18A2 expression vector .
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC18A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 55.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 225128569). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VMAT2 Antibody (OTI9E11)

• MGC120477
• MNAT
• monoamine neurotransmitter transporter
• Monoamine transporter
• SLC18A2
• solute carrier family 18 (vesicular monoamine), member 2
• Solute carrier family 18 member 2
• SVAT
• SVMT
• SVMTMGC120478
• synaptic vesicular amine transporter
• VAT2
• VAT2MGC26538
• vesicle monoamine transporter type 2
• vesicle monoamine/H+ antiporter
• Vesicular amine transporter 2
• VMAT2

Background

The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs,including reserpine and tetrabenazine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VMAT2 Antibody (NBP1-47977)(1)

Publication using NBP1-47977

• Shin EJ, Duong CX, Nguyen XK, Li Z, Bing G, Bach JH, Park DH, Nakayama K, Ali SF, Kanthasamy AG, Cadet JL, Nabeshima T, Kim HC. Role of oxidative stress in methamphetamine-induced dopaminergic toxicity mediated by protein kinase C delta. Behav Brain Res;232(1):98-113. 2012-04-09 [PMID: 22512859] (WB, Mouse)

Review for VMAT2 Antibody (NBP1-47977) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Samuel Jones WB Mouse 08/19/2021

Summary

• Application: Western Blot
• Sample Tested: Brain tissue
• Species: Mouse
• Lot: OTI9E11

Bioinformatics

• Gene Symbol: SLC18A2
• Entrez:
  • 6571(Human)
• Uniprot:
  • Q05940()