BCMA/TNFRSF17 Antibody [Unconjugated]

Images

 
U266 human myeloma cell line was stained with Goat Anti-Human/Primate BCMA/TNFRSF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF193, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
View Available Formulations

BCMA/TNFRSF17 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human BCMA/TNFRSF17
Met1-Ala54
Accession # Q6PE46
Specificity
Detects human BCMA in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhTACI, and recombinant mouse BCMA is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF17
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human BCMA/TNFRSF17 Antibody (Catalog # AF193)
ELISA Detection: Human BCMA/TNFRSF17 Biotinylated Antibody (Catalog # BAF193)
Standard: Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC)
Reviewed Applications
Read 1 Review rated 5
using
AF193 in the following applications:

Publications
Read Publications using
AF193 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCMA/TNFRSF17 Antibody [Unconjugated]

  • B cell maturation antigen
  • B-cell maturation protein
  • BCMA
  • BCMAtumor necrosis factor receptor superfamily member 17
  • BCMB-cell maturation factor
  • CD269 antigen
  • CD269
  • TNFRSF13A
  • TNFRSF17
  • tumor necrosis factor receptor superfamily, member 17

Background

BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56516
Species: Hu, Mu
Applications: WB, IHC, IHC-P
7537-BF/CF
Species: Hu
AF1162
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
H00005478-M01
Species: Hu
Applications: WB, ELISA
NBP1-30032
Species: Hu, Mu, Bv, Xp
Applications: WB, IHC, IHC-Fr
7270-IL/CF
Species: Hu
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P, KO
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB100-1199
Species: Hu, Mu, Rt, Po
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-01801
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-69750
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for BCMA/TNFRSF17 Antibody (AF193)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Flow Cytometry
(1)
IHC-Fr
(1)
IHC-P
(2)
Neutralization
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF193 Applications Species
DX Bu, R Singh, EE Choi, M Ruella, S Nunez-Cruz, KG Mansfield, P Bennett, N Barton, Q Wu, J Zhang, Y Wang, L Wei, S Cogan, T Ezell, S Joshi, KJ Latimer, B Granda, WR Tschantz, RM Young, HA Huet, CJ Richardson, MC Milone Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma Oncotarget, 2018;9(40):25764-25780. 2018 [PMID: 29899820] (IHC-P, Human) IHC-P Human
Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2016;30(1):163-72. 2016 [PMID: 26139429] (Neutralization, Human) Neutralization Human
Carpenter, Robert O, Evbuomwan, Moses O, Pittaluga, Stefania, Rose, Jeremy J, Raffeld, Mark, Yang, Shicheng, Gress, Ronald E, Hakim, Frances, Kochenderfer, James N B-cell Maturation Antigen is a Promising Target for Adoptive T-cell Therapy of Multiple Myeloma. Clin Cancer Res, 2013;19(0):2048-60. 2013 [PMID: 23344265] (IHC-P, Human) IHC-P Human
Daridon C, Devauchelle V, Hutin P, Le Berre R, Martins-Carvalho C, Bendaoud B, Dueymes M, Saraux A, Youinou P, Pers JO Aberrant expression of BAFF by B lymphocytes infiltrating the salivary glands of patients with primary Sjögren&#039;s syndrome. Arthritis Rheum., 2007;56(4):1134-44. 2007 [PMID: 17393395] (IHC-Fr, Human) IHC-Fr Human
Tai YT, Li XF, Breitkreutz I, Song W, Neri P, Catley L, Podar K, Hideshima T, Chauhan D, Raje N, Schlossman R, Richardson P, Munshi NC, Anderson KC Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment. Cancer Res., 2006;66(13):6675-82. 2006 [PMID: 16818641] (Flow Cytometry, Human) Flow Cytometry Human

Review for BCMA/TNFRSF17 Antibody (AF193) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF193:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 01/05/2015
View

Summary

ApplicationELISA
Sample TestedSee PMIC 22804669
SpeciesHuman

Blocking

Blocking DetailsSee PMIC 22804669

Details

Detection NotesSee PMIC 22804669

Comments

CommentsPublished: See PMIC 22804669

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BCMA/TNFRSF17 Antibody (AF193) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional BCMA/TNFRSF17 Products

Bioinformatics Tool for BCMA/TNFRSF17 Antibody (AF193)

Discover related pathways, diseases and genes to BCMA/TNFRSF17 Antibody (AF193). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BCMA/TNFRSF17 Antibody (AF193)

Discover more about diseases related to BCMA/TNFRSF17 Antibody (AF193).
 

Pathways for BCMA/TNFRSF17 Antibody (AF193)

View related products by pathway.

PTMs for BCMA/TNFRSF17 Antibody (AF193)

Learn more about PTMs related to BCMA/TNFRSF17 Antibody (AF193).

Blogs on BCMA/TNFRSF17

There are no specific blogs for BCMA/TNFRSF17, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol TNFRSF17
Uniprot