Bcl-xL Antibody

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Western Blot: Bcl-Xl Antibody [NB110-89469] - Detection of BCL2L1 / Bcl-Xl in whole cell lysate of A431 cell line using NB110-89469.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Fe, Ha, Rb, ShSpecies Glossary
Applications WB, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Bcl-xL Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of human BCL2L1 (within residues 1-50). [Swiss-Prot# Q07817]
Localization
Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity).
Specificity
There are three known isoforms of Bcl-X; Bcl-X(S), Bcl-X(beta), and Bcl-X(L). The immunogen used to create this has 100% identity to all three isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
Application Notes
This BCL2L1 antibody is useful for Western blot, where a band is seen ~26 kDa. This antibody is not useful for ICC/IF.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
A-431 Lysate (NB820-59461)
Bcl-xL Lysate (NBL1-07946)

Reactivity Notes

Human, hamster, canine, sheep, porcine, feline, rabbit, rat and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bcl-xL Antibody

  • Apoptosis regulator Bcl-X
  • bcl2-L-1
  • BCL2-like 1
  • bcl-2-like protein 1
  • BCLXBCL2LBcl-X
  • BclxL
  • Bcl-xL
  • BCL-XL/S
  • BCLXS
  • bcl-xS
  • DKFZp781P2092

Background

BCL2L1 (BCL2-like 1) is a member of BCL-2 protein family and proteins encoded by this gene are located at the outer mitochondrial membrane, where they have been shown to control outer mitochondrial membrane channel (VDAC) opening. VDAC has the ability to regulate mitochondrial membrane potential which is critical to regulation of ROS production as well as release of cytochrome c by mitochondria, both of which are the potent inducers of cellular apoptosis. BCL2L1 also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. There are two alternatively spliced transcript variants, which encode distinct isoforms, and the longer isoform Bcl-X(L) acts as an apoptotic inhibitor whereas the shorter form Bcl-Xl acts as an apoptotic activator. Bcl-X(S) is expressed at high levels in cells undergoing high rate of turnover, such as developing lymphocytes. In contrast, Bcl-Xl is found in tissues containing long-lived postmitotic cells, such as adult brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BCL2L1
Entrez
Uniprot