Bcl-xL Antibody [DyLight 405]

Product Discontinued

Bcl-xL Antibody [DyLight 405] Summary

• Immunogen: Synthetic peptide made to an N-terminal portion of human BCL2L1 (within residues 30-70). [Swiss-Prot# Q07817]
• Localization: Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity).
• Specificity: There are three known isoforms of Bcl-X; Bcl-X(S), Bcl-X(beta), and Bcl-X(L). The immunogen used to create this has 100% identity to all three isoforms.
• Predicted Species: Porcine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL2L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: This BCL2L1 antibody is useful for Western blot, where a band is seen ~26 kDa. This antibody is not useful for ICC/IF.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bcl-xL Antibody [DyLight 405]

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

BCL2L1 (BCL2-like 1) is a member of BCL-2 protein family and proteins encoded by this gene are located at the outer mitochondrial membrane, where they have been shown to control outer mitochondrial membrane channel (VDAC) opening. VDAC has the ability to regulate mitochondrial membrane potential which is critical to regulation of ROS production as well as release of cytochrome c by mitochondria, both of which are the potent inducers of cellular apoptosis. BCL2L1 also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. There are two alternatively spliced transcript variants, which encode distinct isoforms, and the longer isoform Bcl-X(L) acts as an apoptotic inhibitor whereas the shorter form Bcl-X(L) acts as an apoptotic activator. Bcl-X(S) is expressed at high levels in cells undergoing high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2L1