Bcl-xL Antibody

Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56105] - FFPE section of dog ischemic brain cortex stained for Bcl-X expression using this antibody at 1:2000. At 2 hr post ischemia, Bcl-X staining was seen in the dying neurons that had morphological features of apoptosis (arrows). In contrast, mophologically normal appearing neurons lacked Bcl-X staining (arrowheads). Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56105] - FFPE human tissue sections stained for Bcl-X expression using this antibody at 1:2000. (A) Cortex from the brain of an Alzheimer's disease patient. The degenerating neurons are positive for BclX expression, the intact neurons are not. (B) Normal bone marrow. The cells positive for Bcl-X expression appear to be of erythropoetic lineage. Hematoxylin-eosin counterstain.

Product Discontinued

Bcl-xL Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 61-79 (DSPAVNGATAHSSSLDARE) of human Bcl-XL was used as immunogen; GenBank no. CAA80661.1. The immunogen sequence is 94% conserved in Bcl-XS.
• Specificity: Bcl-XL+S
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL2L1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Control:

  Bcl-xL Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for Bcl-xL Antibody

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. NB100-56105 recognizes Bcl-XL and Bcl-XS. Human Bcl-XL is a 233 amino acid protein; GenBank no. CAA80661.1. Human Bcl-XS is a 170 amino acid protein; GenBank no. CAA80662.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bcl-xL Antibody (NB100-56105)(1)

Publication using NB100-56105

• Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (WB, IHC-P, Canine, Mouse, Rat)
  
  Details:
  Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne

Bioinformatics

• Gene Symbol: BCL2L1
• Entrez:
  • 598(Human)
  • 12048(Mouse)
  • 24888(Rat)