Bcl-2 Antibody (121529)

Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse Bcl-2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB810) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Bcl-2 Minus C-Terminus (Catalog # 827-BC) (2 ng) was included. A specific band for Bcl-2 was detected at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

TUNEL assay of cardiac myocyte apoptosis in the hearts. (A) Quantification of TUNEL-positive nuclei n=3. Representative qPCR (B) and western blot (C) analysis (original blots/gels are presented in Supplementary 2 Fig.7-9) of apoptosis-related mRNA and protein, n=3, Chemiluminescence signals were visualized and cropped using Protein Simple Compass software. The whole cell lysates were used for western blot. The data are presented as the mean SEM; *P<0.05; **P<0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/40188200/), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Mu
• Applications: WB
• Clone: 121529
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Bcl-2 Antibody (121529) Summary

• Immunogen: E. coli-derived recombinant mouse Bcl-2
• Specificity: Detects mouse Bcl‑2 Minus C-Terminus in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: BCL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bcl-2 Antibody (121529)

• apoptosis regulator Bcl-2
• B-cell CLL/lymphoma 2
• Bcl2
• Bcl-2

Background

The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bcl-2 is a 26 kD protein associated with cell survival. Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis in a number of cells. The ratio of Bcl-2 to other Bcl-2 family members and subcellular localization are believed to modulate the apoptotic process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bcl-2 Antibody (MAB810)(1)

Publication using MAB810

• Park EJ, Zhao YZ, Kim YC, Sohn DH Bakuchiol-induced caspase-3-dependent apoptosis occurs through c-Jun NH2-terminal kinase-mediated mitochondrial translocation of Bax in rat liver myofibroblasts. Eur. J. Pharmacol., 2007-01-23;559(2):115-23. 2007-01-23 [PMID: 17292878] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: BCL2