BBOX1 Antibody - BSA Free

Western Blot: BBOX1 Antibody [NBP1-32327] - Sample(30 ug of whole cell lysate)A:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: BBOX1 Antibody [NBP1-32327] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327] - mouse kidney. BBOX1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: BBOX1 Antibody [NBP1-32327] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327] - Ca922 xenograft. BBOX1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BBOX1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human BBOX1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BBOX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BBOX1 Antibody - BSA Free

• 2-oxoglutarate dioxygenase 1
• 2-oxoglutarate dioxygenase
• BBHgamma-butyrobetaine
• BBOXEC 1.14.11.1
• butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetainehydroxylase) 1
• Gamma-BBH
• gamma-butyrobetaine dioxygenase
• Gamma-butyrobetaine hydroxylase
• Gamma-butyrobetaine
• G-BBH

Background

This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for BBOX1 Antibody (NBP1-32327)(1)

Publication using NBP1-32327

• Terry EE, Zhang X, Hoffmann C et al. Transcriptional profiling reveals extraordinary diversity among skeletal muscle tissues Elife 2018-05-29 [PMID: 29809149] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: BBOX1