Calcineurin B Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse brain tissue,HT‑29 human colon adenocarcinoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and TS1 mouse helper T cell line. PVDF membrane was ...read more
Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Calcineurin B at approximately 24 kDa (as indicated) using 2.5 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Calcineurin B Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Calcineurin B
Accession # P63098
Specificity
Detects human, mouse, and rat Calcineurin B in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP3R1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF1348 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calcineurin B Antibody [Unconjugated]

  • Calcineurin B
  • calcineurin subunit B type 1
  • CALNB1
  • CNA2
  • Cnb1
  • MCIP1
  • PP2B beta 1
  • PPP3R1
  • Protein phosphatase 2B regulatory subunit 1
  • protein phosphatase 2B regulatory subunit B alpha
  • protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I)
  • protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I)
  • protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
  • Protein phosphatase 3 regulatory subunit B alpha isoform 1
  • protein phosphatase 3, regulatory subunit B, alpha
  • type I (19kDa)

Background

Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 1,900 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin (1). Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory (2). It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation (3). Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity (4). Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy (5) and graft versus host disease in organ transplantation (6).

  1.  Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
  2. Zeng H. et al. (2001) Cell 107:617.
  3. Okamura, H. et al. (2000) Molecular Cell 6:539.
  4. Liu, J. et al. (1992) Biochemistry 31:3896.
  5. Molkentin, J.D. (2000) Circulation Research 87:731.
  6. Sanquer, S.A. et al. (2004) Transplantation 77:854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1348
Species: Hu, Mu, Rt
Applications: WB, Simple Western

Publications for Calcineurin B Antibody (AF1348)(4)

We have publications tested in 3 confirmed species: Human, Rat, Hamster.

We have publications tested in 2 applications: IHC, Western Blot.


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IHC
(1)
Western Blot
(3)
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Filter By Species
Human
(1)
Rat
(2)
Hamster
(1)
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Video Protocols

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Bioinformatics

Gene Symbol PPP3R1
Uniprot