Calcineurin A Antibody (302202)

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 302202
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Calcineurin A Antibody (302202) Summary

• Immunogen: Bovine brain Calcineurin holoenzyme (A and B subunits)
• Specificity: Detects human, mouse, and rat Calcineurin A.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: PPP3CA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calcineurin A Antibody (302202)

• calcineurin A alpha
• Calcineurin A
• Calmodulin-dependent calcineurin A subunit alpha isoform
• CALN
• CALNA
• CALNAcatalytic subunit, alpha isoform
• CAM-PRP catalytic subunit
• CNA
• CNA1
• CNA1CALNA1
• EC 3.1.3.16
• PP2B
• PP3CA
• PPP2B
• PPP2BCCN1
• PPP3CA
• protein phosphatase 2B, catalytic subunit, alpha isoform
• protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
• protein phosphatase 3, catalytic subunit, alpha isozyme
• serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin A, also known as PP2B and PPP3CA, is the 59 kDa catalytic subunit of the calcium/calmodulin-dependent protein phosphatase. When activated by calcium in the presence of the regulatory B subunit and calmodulin, Calcineurin A selectively removes phosphates from serine and threonine residues on target proteins. Although ubiquitously expressed, Calcineurin levels are highest in brain, where the phosphatase plays a role in the formation and retention of memories. Calcineurin activity is inhibited by the immunosuppressant drug cyclosporine A bound to cyclophilins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calcineurin A Antibody (MAB2839)(5)

Publications using MAB2839

• Ajay Mishra, Bénédicte Oulès, Angela Oliveira Pisco, Tony Ly, Kifayathullah Liakath-Ali, Gernot Walko et al. A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis eLife 10/18/2017 [PMID: 29043977]
• Ahmad F. Alghanem, Emma L. Wilkinson, Maxine S. Emmett, Mohammad A. Aljasir, Katherine Holmes, Beverley A. Rothermel et al. RCAN1.4 regulates VEGFR-2 internalisation, cell polarity and migration in human microvascular endothelial cells Angiogenesis 8/1/2017 [PMID: 28271280]
• Ahmad F. Alghanem, Emma L. Wilkinson, Maxine S. Emmett, Mohammad A. Aljasir, Katherine Holmes, Beverley A. Rothermel et al. RCAN1.4 regulates VEGFR-2 internalisation, cell polarity and migration in human microvascular endothelial cells Angiogenesis 2017-08-01 [PMID: 28271280] (Western Blot, Human)
• Gruol DL, Puro A, Hao C et al. Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6. J Neuroimmunol. 9/2/2011 [PMID: 21890220]
• Planel E, Tatebayashi Y, Miyasaka T, Liu L, Wang L, Herman M, Yu WH, Luchsinger JA, Wadzinski B, Duff KE, Takashima A Insulin dysfunction induces in vivo tau hyperphosphorylation through distinct mechanisms. J. Neurosci., 2007-12-12;27(50):13635-48. 2007-12-12 [PMID: 18077675] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PPP3CA