Human Bax ELISA Kit (Colorimetric)

ELISA: Bax ELISA Kit (Colorimetric) [NBP2-61302] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Human Bax ELISA Kit (Colorimetric) Summary

• Description: The Bax (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The Bax (human), EIA kit provides sensitive results - detect picogram levels of protein compared to microgram levels in Western blot and extend the utility of your precious samples.
• Standard Curve Range: 62.5 - 2,000 pg/ml
• Sensitivity: 10.1 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: Mean 9.6%
• Intra-Assay: Mean 5.4%
• Spike Recovery: Cell Lysis Buffer 4 - 100.6% 62,500 cells/mL - 98.6%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BAX

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human Bax ELISA Kit (Colorimetric)

• apoptosis regulator BAX
• Bax
• BCL2-associated X protein
• Bcl2-L-4
• BCL2L4bcl2-L-4
• Bcl-2-like protein 4

Background

Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Bax ELISA Kit (NBP2-61302). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BAX