Ku70's Roles in Double Strand and Mismatch DNA Repair

Thu, 05/24/2012 - 12:11

Ku70 is a 70 kDa protein that was shown to be involved in multiple cellular pathways, mainly involving DNA repair and recombination. Among these are the non-homologus end repairs of DNA double strand breaks. Ku70 was first identified as an autoantigen in the serum of patients with Scleroderma Ploymyosistosis Syndrome (1). Auto antibodies against Ku70 were also found in other autoimmune diseases such as Scleroderma and Systemic Lupus Erythromatosus.

Immunohistochemistry-Paraffin: Ku70 Antibody

Ku proteins are multifunctional proteins that possess deubiquitylation activity and play a key role in DNA repair and transcriptional regulation (2). Substantial biochemical evidence also indicates that various proteins physically interact with the Ku complex. For example, Ku70/80 interacts with both the protein and RNA components of human telomerase, suggesting that the Ku complex is involved in telomere maintenance in eukaryotes (3), these complexes have been shown to inhibit apoptosis through an association with the proapoptotic factor Bax, Interactions between Ku70 and p18-cyclin E, and Ku70 and Bax, provide a balance between apoptosis and cell survival in response to genotoxic stress (4). Interaction of Ku70 proteins with mismatch repair (MMR) proteins has been detected in human cells by using antibodies against Ku70. Interactions were detected by anti-Ku70 antibody either by immunoprecipitation or by IHC studies. These data suggest a strong role for MMR protein involvement in the repair of double strand breaks in DNA via Ku70 (5).

  1. PMID: 19448404
  2. PMID: 0755664
  3. PMID: 15824061
  4. PMID: 17325036
  5. PMID: 21075794

Novus Biologicals offers Ku70 reagents for your research needs including:

Blog Topics