Bax Antibody (YTH-2D2) [Unconjugated]

Product Discontinued

Bax Antibody (YTH-2D2) [Unconjugated] Summary

• Immunogen: Human Bax
  aa 3-16
• Specificity: Anti-Bax (Clone YTH-2D2) cross reacts with human Bax.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAX
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: Store at 4 °C.
• Buffer: This antibody is provided as purified immunoglobulin from mouse ascites in 1X PBS containing 0.01% sodium azide.
• Preservative: 0.01% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bax Antibody (YTH-2D2) [Unconjugated]

• apoptosis regulator BAX
• Bax
• BCL2-associated X protein
• Bcl2-L-4
• BCL2L4bcl2-L-4
• Bcl-2-like protein 4

Background

The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bax has been shown to redistribute from the cytosol to the mitochondria during apoptosis, and overexpression of Bax can accelerate cell death. Coregulation of Bax dimer formation and intracellular localization are associated with Bax conformational changes. Anti-Bax YTH-2D2 is specific for human Bax and does not crossreact with mouse or rat Bax. When used in conjunction with the anti-Bax YTH-6A7 (Catalog # 2281-MC), which recognizes only detergent treated Bax, the researcher may selectively identify the activated form of Bax.

1. Suzuki, M. et al. 2000, Cell 103:645-54.
2. Hsu, Y.-T. et al. 1997, Proc. Natl. Acad. Sci. USA 94:3668-3672.
3. Hsu, Y.-T. and R.J. Youle. 1997, J. Biol. Chem. 272:13829-13834.
4. Neuchushtan, A. et al. 1999, EMBO J. 18:2330-2341.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bax Antibody (2282-MC)(8)

Publications using 2282-MC

• K Ibraheem, AMA Yhmed, MM Nasef, NT Georgopoul TRAF3/p38-JNK Signalling Crosstalk with Intracellular-TRAIL/Caspase-10-Induced Apoptosis Accelerates ROS-Driven Cancer Cell-Specific Death by CD40 Cells, 2022-10-18;11(20):. 2022-10-18 [PMID: 36291141] (Western Blot, Human)
• A redox state-dictated signalling pathway deciphers the malignant cell specificity of CD40-mediated apoptosis Oncogene, 2016-11-21;0(0):. 2016-11-21 [PMID: 27869172] (Western Blot, Human)
• Liu FT, Agrawal SG, Movasaghi Z, Wyatt PB, Rehman IU, Gribben JG, Newland AC, Jia L Dietary flavonoids inhibit the anticancer effects of the proteasome inhibitor bortezomib. Blood, 2008-07-16;112(9):3835-46. 2008-07-16 [PMID: 18633129] (Western Blot, Human)
• Agrawal SG, Liu FT, Wiseman C, Shirali S, Liu H, Lillington D, Du MQ, Syndercombe-Court D, Newland AC, Gribben JG, Jia L Increased proteasomal degradation of Bax is a common feature of poor prognosis chronic lymphocytic leukemia. Blood, 2007-12-26;111(5):2790-6. 2007-12-26 [PMID: 18160666] (Western Blot, Human)
• Oliver L, Mahe B, Gréé R, Vallette FM, Juin P HA14-1, a small molecule inhibitor of Bcl-2, bypasses chemoresistance in leukaemia cells. Leuk. Res., 2007-01-16;31(6):859-63. 2007-01-16 [PMID: 17224180] (Western Blot, Human)
• Liu FT, Goff LK, Hao JH, Newland AC, Jia L Increase in the ratio of mitochondrial Bax/Bcl-XL induces Bax activation in human leukemic K562 cell line. Apoptosis, 2004-05-01;9(3):377-84. 2004-05-01 [PMID: 15258470] (Immunoprecipitation, Human)
• Hao JH, Yu M, Liu FT, Newland AC, Jia L Bcl-2 inhibitors sensitize tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by uncoupling of mitochondrial respiration in human leukemic CEM cells. Cancer Res., 2004-05-15;64(10):3607-16. 2004-05-15 [PMID: 15150119] (Western Blot, Human)
• Jia L, («e͍֗R, Patwari Y, Srinivasula SM, Newland AC, Fernandes-Alnemri T, Alnemri ES, Kelsey SM Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis. ÔÏŒ?RoPJZ¤ ÁÅù$âзšÇ®å:AgÁž´²¾»<³“ÕEN½;ò°rÓ¦Ò°ލ/fø_kŠ¾ûÎnîålŸT¯¤:Qº3I`¨Ò, 2001-08-09;20(35):4817-26. 2001-08-09 [PMID: 11521193] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BAX