BANP Antibody

Images

 
Western Blot: BANP Antibody [NBP1-76295] - mouse kidney tissue lysate with BANP antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry: BANP Antibody [NBP1-76295] - Immunohistochemistry of BANP in human kidney tissue with BANP antibody at 10 ug/mL.
Immunofluorescence: BANP Antibody [NBP1-76295] - Immunofluorescence of BANP in human kidney tissue with BANP antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ICC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BANP Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the center of human BANP. The immunogen is located within amino acids 300 - 350 of BANP.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BANP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 20 ug/ml
  • Immunocytochemistry 20 ug/ml
Control Peptide
BANP Peptide (NBP1-76295PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BANP Antibody

  • BEN domain containing 1
  • BEND1BEN domain-containing protein 1
  • BTG3 associated nuclear protein
  • Btg3-associated nuclear protein
  • DKFZp761H172
  • FLJ10177
  • FLJ20538
  • protein BANP
  • SMAR1Scaffold/matrix-associated region-1-binding protein
  • SMARBP1

Background

BANP was initially identified as a binding protein to BTG3 in a yeast two-hybrid screen. BANP acts as a tumor suppressor by stabilizing p53 expression and leading to cell cycle arrest. p53 in turn binds to upstream elements of the BANP promoter, thereby forming a feedback loop. BANP is down-regulated in advanced stages of human breast cancer, and its overexpression in breast cancer cell lines inhibits their ability to metastasize by modulating TGF-beta signaling. Furthermore, BANP can modulate NF-kappaB transactivation and can inhibit tumorigenesis by regulating NF-kappaB target genes. Recent experiments have shown that BANP can also repress HIV-1 LTR mediated transcription by tethering the LTR matrix attachment region to nuclear matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BANP
Uniprot