BAK Antibody

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Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BAK Antibody Summary

Immunogen
Peptide with sequence ASGQGPGPPRQE-C corresponding to N-Terminus according to NP_001179.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BAK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/mL
  • Peptide ELISA Detection limit 1:32000
Control
BAK Overexpression Lysate
BAK Knockout HeLa Cell Lysate
Publications
Read Publication using NB100-893.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAK Antibody

  • Apoptosis regulator BAK
  • BAK
  • BAK1
  • BAK-LIKE
  • BAKMGC3887
  • BCL2-antagonist/killer 1
  • BCL2L7
  • bcl2-L-7
  • BCL2L7bcl-2 homologous antagonist/killer
  • BCL2-like 7 protein
  • Bcl-2-like protein 7
  • CDN1
  • MGC117255
  • pro-apoptotic protein BAK

Background

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl2 family members, among others, play an important role.The Bcl2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of holologous proteins which function in the regulation of apoptosis. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relativeratios of the different Bcl2 family proteins. Bak is a 211 aa protein (24kD) which has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. Bak has been localized by immunohistochemistry to the cytosol of cells and stains in a punctate pattern characteristic of other bcl2 family proteins, which have been localized to intracellular organelles. Immunohistochemical studies further show that Bak expression is widespread, and may show some correlation with the apoptopic state of cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAK Antibody (NB100-893)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on BAK.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Bioinformatics

Gene Symbol BAK1
Entrez
Uniprot