Bag-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining in human thyroid gland and pancreas tissues using anti-BAG1 antibody. Corresponding BAG1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining of human breast shows moderate positivity in glandular cells.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining of human thyroid gland shows high expression.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Bag-1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Bag-1 Protein (NBP1-89547PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Bag-1 Antibody - BSA Free

• BAG family molecular chaperone regulator 1
• Bag1
• Bag-1
• Bcl-2 associating athanogene-1 protein
• Bcl-2-associated athanogene 1
• BCL2-associated athanogene
• Bcl-2-binding protein
• glucocortoid receptor-associated protein RAP46,46-KD
• HAP
• RAP46

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAG1