Bag-1 Antibody (RM310)

Western Blot: Bag-1 Antibody (RM310) [NBP2-77409] - Western Blot of HeLa cell lysate, using NBP2-77409.

Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded BAG-1L overexpressing LNCaP cell section using NBP2-77409.

Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using NBP2-77409.

Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using NBP2-77409.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: RM310
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Bag-1 Antibody (RM310) Summary

• Description: Novus Biologicals Rabbit Bag-1 Antibody (RM310) (NBP2-77409) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A peptide corresponding to residues near the N-terminus of human Bag-1
• Specificity: This antibody reacts to human Bag-1L. It does not cross react to Bag-1M or Bag-1S.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BAG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Western Blot 1:1000 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Bag-1 Antibody (RM310)

• BAG family molecular chaperone regulator 1
• Bag1
• Bag-1
• Bcl-2 associating athanogene-1 protein
• Bcl-2-associated athanogene 1
• BCL2-associated athanogene
• Bcl-2-binding protein
• glucocortoid receptor-associated protein RAP46,46-KD
• HAP
• RAP46

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAG1