Bag-1 Antibody (1E4B5) - BSA Free

Images

 
Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against human BAG1 (AA: 219-346) recombinant protein. (Expected MW is 40.6 kDa)
Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green) and negative control (red).
Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against HEK293 (1) and BAG1 (AA: 219-346)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Bag-1 Antibody (1E4B5) [NBP2-61698] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
1E4B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Bag-1 Antibody (1E4B5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human BAG1 (AA: 219-346) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BAG1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:2000
Theoretical MW
38.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Bag-1 Antibody (1E4B5) - BSA Free

  • BAG family molecular chaperone regulator 1
  • Bag1
  • Bag-1
  • Bcl-2 associating athanogene-1 protein
  • Bcl-2-associated athanogene 1
  • BCL2-associated athanogene
  • Bcl-2-binding protein
  • glucocortoid receptor-associated protein RAP46,46-KD
  • HAP
  • RAP46

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on Bag-1.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BAG1