Bad [p Ser112] Antibody - BSA Free

Western Blot: Bad [p Ser112] Antibody [NB100-81807] - Analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112)

Immunohistochemistry-Paraffin: Bad [p Ser112] Antibody [NB100-81807] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81807 (left) or the same antibody preincubated with blocking peptide(right).

Immunohistochemistry-Paraffin: Bad [p Ser112] Antibody [NB100-81807] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81807(left) or the same antibody preincubated with blocking peptide(right).

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Bad [p Ser112] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Bad [p Ser112] Antibody - BSA Free (NB100-81807) is a polyclonal antibody validated for use in IHC and WB. Anti-Bad Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from mouse Bad around the phosphorylation site of serine 112 (H-S-SP-Y-P).
• Modification: p Ser112
• Specificity: Detects endogenous levels of Bad only when phosphorylated at serine 112.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Bad Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Bad [p Ser112] Antibody - BSA Free

• Bad
• BBC2
• BBC6
• bcl2 antagonist of cell death
• BCL2-antagonist of cell death protein
• BCL2-associated agonist of cell death
• Bcl-2-binding component 6
• BCL2-binding component 6
• BCL2-binding protein
• BCL2L8
• Bcl2-L-8
• BCL2L8bcl2-L-8
• Bcl-2-like protein 8
• BCL-X/BCL-2 binding protein
• Bcl-XL/Bcl-2-associated death promoter

Background

Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bad Antibody (NB100-81807)(1)

Publication using NB100-81807

• Mega Tiber P, Kocyigit Sevinc S, Kilinc O, Orun O Biological effects of whole Z.Officinale extract on chronic myeloid leukemia cell line K562. Gene. 2019-04-15 [PMID: 30684525] (WB, Human)
  
  Details:
  Citation using the Azide free version of this antibody.

Bioinformatics

• Gene Symbol: BAD
• Entrez:
  • 572(Human)
  • 64639(Rat)
• Uniprot:
  • Q92934(Human)