B7-H2/ICOSLG Antibody (987602) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083) followed by APC-conjugated Anti-Rat IgG Secondary Antibody(Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins.

Recombinant Mouse B7-H2 (Catalog # 8127-B7) induces IL-4 secretion in the D10.G4.1 mouse helper T cell line in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) in a dose-dependent manner (orange line), as measured by the Mouse IL-4 Quantikine ELISA Kit (Catalog # M4000B). Under these conditions, IL-4 secretion elicited by B7-H2 (2.5 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). The ND50 is typically 0.3-1.8 μg/mL.

In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2 (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). Rat IgG2A Isotype Control (Catalog # MAB006) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with Mouse Anti-Human IgG Fc APC‑conjugated Monoclonal Antibody (Catalog # FAB110A).

• Reactivity: Mu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 987602
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

B7-H2/ICOSLG Antibody (987602) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
  Met1-Lys279
  Accession # Q9JHJ8
• Specificity: Detects mouse B7-H2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction 0.5 ug/10^6 cells
  • CyTOF-ready
  • Neutralization

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H2/ICOSLG Antibody (987602) [Unconjugated]

• B7H2
• B7-H2
• B7H2B7 homolog 2
• B7-H2B7-related protein 1
• B7RP-1
• B7RP1B7-like protein Gl50
• B7RP-1LICOS
• CD275 antigen
• CD275
• GL50
• GL50transmembrane protein B7-H2 ICOS ligand
• ICOSL
• ICOS-L
• ICOSLG
• inducible T-cell co-stimulator ligand
• KIAA0653ICOS ligand

Background

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9JHJ8()