| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF |
| Clone | C80/1608 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Recombinant human full-length CD80 protein |
| Localization | Cell Surface |
| Protein/Peptide Type | Recombinant Protein |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD80 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA (For coating order Ab without BSA) |
| Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54307AF350 | |
| Alexa Fluor 405 | NBP2-54307AF405 | |
| Alexa Fluor 488 | NBP2-54307AF488 | |
| Alexa Fluor 532 | NBP2-54307AF532 | |
| Alexa Fluor 594 | NBP2-54307AF594 | |
| Alexa Fluor 647 | NBP2-54307AF647 | |
| Alexa Fluor 700 | NBP2-54307AF700 | |
| Alexa Fluor 750 | NBP2-54307AF750 | |
| Allophycocyanin | NBP2-54307APC | |
| Allophycocyanin/Cy7 | NBP2-54307APCCY7 | |
| Biotin | NBP2-54307B | |
| DyLight 350 | NBP2-54307UV | |
| DyLight 405 | NBP2-54307V | |
| DyLight 488 | NBP2-54307G | |
| DyLight 550 | NBP2-54307R | |
| DyLight 594 | NBP2-54307DL594 | |
| DyLight 650 | NBP2-54307C | |
| DyLight 680 | NBP2-54307FR | |
| DyLight 755 | NBP2-54307IR | |
| FITC | NBP2-54307F | |
| HRP | NBP2-54307H | |
| Janelia Fluor 549 | NBP2-54307JF549 | |
| Janelia Fluor 646 | NBP2-54307JF646 | |
| PE | NBP2-54307PE | |
| PE/Atto594 | NBP2-54307PEATT594 | |
| PE/Cy5.5 | NBP2-54307PECY55 | |
| PE/Cy7 | NBP2-54307PECY7 | |
| PerCP | NBP2-54307PCP |
Diseases for B7-1/CD80 Antibody (NBP2-53176)Discover more about diseases related to B7-1/CD80 Antibody (NBP2-53176).
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PTMs for B7-1/CD80 Antibody (NBP2-53176)Learn more about PTMs related to B7-1/CD80 Antibody (NBP2-53176).
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CD80: A co-stimulator of T cell activation CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re... Read full blog post. |
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| Gene Symbol | CD80 |
![Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize2/B7-2-CD86-Antibody-(BU63)-Knockout-Validated-NBP2-25208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize2/B7-2-CD86-Antibody-(BU63)-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/fullsize2/B7-2-CD86-Antibody-(BU63)-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/fullsize2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/fullsize2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/fullsize2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79843-img0005.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Flow-(Intracellular)-NB100-524-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - CD40-transfected L cells stained.](http://images.novusbio.com/fullsize2/CD40-TNFRSF5-Antibody-(G28-5)-Immunocytochemistry-Immunofluorescence-DDX0130P-100-img0002.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (G28-5) [DDX0130P-100] - Human cervical tissue. a: normal cervical epithelium. b: cervical intraepithelial neoplasia. c: squamous cell carcinoma.](http://images.novusbio.com/fullsize2/CD40-TNFRSF5-Antibody-(G28-5)-Immunohistochemistry-Paraffin-DDX0130P-100-img0001.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/fullsize2/Doublecortin-Antibody-(3E1)-Western-Blot-NBP1-92684-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/fullsize2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.](http://images.novusbio.com/fullsize2/APC-Antibody-Western-Blot-NB100-91662-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/APC-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91662-img0006.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.](http://images.novusbio.com/fullsize2/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Coagulation-Factor-XIV-Protein-C-Antibody-(JM93-61)-Immunocytochemistry-Immunofluorescence-NBP2-66987-img0002.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human liver tissue using anti-Protein C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Coagulation-Factor-XIV-Protein-C-Antibody-(JM93-61)-Immunohistochemistry-Paraffin-NBP2-66987-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Coagulation-Factor-XIV-Protein-C-Antibody-(JM93-61)-Immunocytochemistry-Immunofluorescence-NBP2-66987-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Immunocytochemistry-NBP1-49045-img0011.jpg)
![Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Immunohistochemistry-Frozen-NBP1-49045-img0015.jpg)
![Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/fullsize2/CD8-alpha-Antibody-(53-6.7)-Flow-Cytometry-NBP1-49045-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/MHC Class I Antibody (OX18)-Immunocytochemistry-Immunofluorescence-NB120-6405-img0009.jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Immunohistochemistry-Paraffin-NB120-6405-img0008.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Flow-Cytometry-NB120-6405-img0007.jpg)
![Flow Cytometry: Mouse, Human IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Image from verified customer review.](http://images.novusbio.com/images2/Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1)-Flow Cytometry-NBP1-43319-img0004.jpg "Flow Cytometry: Mouse, Human IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Image from verified customer review.")