B7-1/CD80 Antibody (2D10.4)

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Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) ...read more
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the FITC conjugate of NBP1-43384. Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (cat. 11-4714) (blue histogram) or ...read more
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE conjugate of NBP1-43384. Analysis of human peripheral blood leukocytes using CD80 PE conjugage. This can be used at less than or equal to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Func
Clone
2D10.4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

B7-1/CD80 Antibody (2D10.4) Summary

Immunogen
The immunogen for this antibody was CD80.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD80
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Functional
Application Notes
The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in functional reported in scientific literature (PMID 15951837)
Publications
Read Publications using NBP1-43384.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for B7-1/CD80 Antibody (2D10.4)

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-1/CD80 Antibody (NBP1-43384)(2)

We have publications tested in 1 application: Func.


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Func
(2)
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Showing Publications 1 - 2 of 2.
Publications using NBP1-43384 Applications Species
Bashuda H, Kimikawa M, Seino K et al. Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates J Clin Invest 2005 Jul [PMID: 15951837] (Func)

Details:
This citation used the FITC version of this antibody.
Func
Bashuda H, Kimikawa M, Seino K et al. Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates J Clin Invest Jun 27 1905 (Func)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP2-00431).
Func

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-43616
PE/Cy5 Labeled NBP2-00123

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Bioinformatics Tool for B7-1/CD80 Antibody (NBP1-43384)

Discover related pathways, diseases and genes to B7-1/CD80 Antibody (NBP1-43384). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD80
Entrez