B7-1/CD80 Antibody (2D10.4)


Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) ...read more
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the FITC conjugate of NBP1-43384. Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (cat. 11-4714) (blue histogram) or ...read more
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE conjugate of NBP1-43384. Analysis of human peripheral blood leukocytes using CD80 PE conjugage. This can be used at less than or equal to ...read more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, Func
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

B7-1/CD80 Antibody (2D10.4) Summary

The immunogen for this antibody was CD80.
IgG1 Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
  • Functional
Application Notes
The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in functional reported in scientific literature (PMID 15951837)
Read Publication using NBP1-43384.

Alternate Names for B7-1/CD80 Antibody (2D10.4)

  • Activation B7-1 antigen
  • B7
  • B71
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80


The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-1/CD80 Antibody (NBP1-43384)(1)

We have publications tested in 1 application: Func.

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Publication using NBP1-43384 Applications Species
Bashuda H, Kimikawa M, Seino K et al. Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates J Clin Invest Jun 27 1905 12:00AM (Func) Func

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP1-43616
FITC Labeled NBP2-00431
PE Labeled NBP1-43841
PE/Cy5 Labeled NBP2-00123

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Blogs on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Product PDFs


Gene Symbol CD80

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