B7-1/CD80 Antibody (2D10.4) - BSA Free

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) orAnti-Human CD80 (B7-1) PE-Cy5 (filled histogram).

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the FITC conjugate of NBP1-43384. Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (cat. 11-4714) (blue histogram) or Anti-Human CD80 (B7-1) FITC (purple histogram). Total viable cells were used for analysis.

Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE conjugate of NBP1-43384. Analysis of human peripheral blood leukocytes using CD80 PE conjugage. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.

• Reactivity: Hu
• Applications: Flow, Func
• Clone: 2D10.4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

B7-1/CD80 Antibody (2D10.4) - BSA Free Summary

• Additional Information: Clone 2D10.4 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD80.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD80
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Functional
• Application Notes: The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in functional reported in scientific literature (PMID 15951837)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for B7-1/CD80 Antibody (2D10.4) - BSA Free

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-1/CD80 Antibody (NBP1-43384)(2)

Publications using NBP1-43384

• Chen Y, Guo TL. Dietary Early Glycation Products Promote the Growth of Prostate Tumors More than Advanced Glycation End-Products through Modulation of Macrophage Polarization Mol Nutr Food Res 2018-12-04 [PMID: 30512230] (FLOW, Human)
• Bashuda H, Kimikawa M, Seino K et al. Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates J Clin Invest 2005-07-01 [PMID: 15951837] (Func)
  
  Details:
  This citation used the FITC version of this antibody.

Bioinformatics

• Gene Symbol: CD80