B7-1/CD80 Antibody (2D10.4) - BSA Free

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Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) ...read more
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the FITC conjugate of NBP1-43384. Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (cat. 11-4714) (blue histogram) or ...read more
Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE conjugate of NBP1-43384. Analysis of human peripheral blood leukocytes using CD80 PE conjugage. This can be used at less than or equal to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Func
Clone
2D10.4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

B7-1/CD80 Antibody (2D10.4) - BSA Free Summary

Additional Information
Clone 2D10.4 was used by HLDA to establish CD designation.
Immunogen
The immunogen for this antibody was CD80.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD80
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Functional
Application Notes
The 2D10.4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in functional reported in scientific literature (PMID 15951837)
Publications
Read Publications using
NBP1-43384 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for B7-1/CD80 Antibody (2D10.4) - BSA Free

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-1/CD80 Antibody (NBP1-43384)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, Func.


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Secondary Antibodies

 

Isotype Controls

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Research Areas for B7-1/CD80 Antibody (NBP1-43384)

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Blogs on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD80