B7-1/CD80 Antibody (111114) [Unconjugated]

B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of mouse colon tissue using Rat Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB740) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB740) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 111114
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

B7-1/CD80 Antibody (111114) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80
  Val38-Asn246
  Accession # Q00609
• Specificity: Detects mouse B7-1/CD80 in direct ELISAs and Western blots. No cross-reactivity with recombinant mouse (rm) rmB7-2, rmB7-H2 and recombinant human (rh) B7-H1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD80
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-1/CD80 Antibody (111114) [Unconjugated]

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma . B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

1. Azuma, M. et al. (1993) Nature 366:76.
2. Freeman, G.J. et al. (1993) Science 262:909.
3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
5. Chen, C. et al. (1994) J. Immunol. 152:4929.
6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-1/CD80 Antibody (MAB740)(4)

Publications using MAB740

• Y Kuninaka, Y Ishida, A Ishigami, M Nosaka, J Matsuki, H Yasuda, A Kofuna, A Kimura, F Furukawa, T Kondo Macrophage polarity and wound age determination Scientific Reports, 2022-11-25;12(1):20327. 2022-11-25 [PMID: 36434083] (IHC, Mouse)
• V Hoghooghi, AL Palmer, A Frederick, Y Jiang, JE Merkens, A Balakrishn, TM Finlay, A Grubb, E Levy, P Gordon, FR Jirik, MD Nguyen, C Schuurmans, F Visser, SE Dunn, SS Ousman Cystatin C Plays a Sex-Dependent Detrimental Role in Experimental Autoimmune Encephalomyelitis Cell Rep, 2020-10-06;33(1):108236. 2020-10-06 [PMID: 33027652] (Western Blot, Mouse)
• SC Garrett-Th, A Massimi, EV Fedorov, JB Bonanno, L Scandiuzzi, B Hillerich, RD Seidel, JD Love, SJ Garforth, C Guha, SC Almo Mechanistic dissection of the PD-L1:B7-1 co-inhibitory immune complex PLoS ONE, 2020-06-04;15(6):e0233578. 2020-06-04 [PMID: 32497097] (ICC, Mouse)
• J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice EBioMedicine, 2018-09-15;35(0):29-39. 2018-09-15 [PMID: 30228093] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CD80
• Uniprot:
  • Q00609()