B220/CD45R Antibody (RA3-6B2)

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Flow Cytometry: B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis of Biotin conjugate of NB100-77420. Staining of BALB/c splenocytes with 0.06 ug of Rat IgG2a kappa Isotype Control Biotin (open histogram) or 0.06 ...read more
Flow Cytometry: B220/CD45R Antibody (RA3-6B2) [NB100-77420] - C57BL/6 mouse splenocytes stained with purified RA3-6B2, followed by anti-rat IgG FITC
Flow (Cell Surface): B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-77420. Staining of CD45R on 10^6 BALB/c mouse splenocytes using 0.25 ug of this antibody. ...read more
Flow (Cell Surface): B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77420. Staining of CD45R on 10^6 BALB/c mouse splenocytes using 0.25 ug of this antibody. ...read more
Flow Cytometry: B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis using the Cy5 conjugate of NB100-77420. Multiple staining of BALB/c splenocytes.
Flow Cytometry: B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-77420. Cell surface CD45R on 10^6 BALB/c mouse splenocytes using 0.25 ug of this antibody. Green histogram ...read more

Product Details

Summary
Reactivity Hu, Mu, FeSpecies Glossary
Applications Flow, Flow-CS, IHC, IHC-Fr, IHC-P, IP
Clone
RA3-6B2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

B220/CD45R Antibody (RA3-6B2) Summary

Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry < 0.25 ug/10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Flow (Cell Surface)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry, It is recommended that the reagent be titrated for optimal performance for each application. Useful in Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation as reported in Literature.
Positive Control
CD45 Lysate (NBL1-14981)
Reviewed Applications
Read 1 Review rated 5
using
NB100-77420 in the following applications:

Publications
Read Publications using
NB100-77420 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for B220/CD45R Antibody (RA3-6B2)

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45R is an isoform of CD45, also known as B220. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B220/CD45R Antibody (NB100-77420)(4)

Review for B220/CD45R Antibody (NB100-77420) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-77420:
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WB
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Shijun Yan
WB Mouse 06/29/2016
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Summary

ApplicationWestern Blot
Sample Testedspleen
SpeciesMouse

Blocking

Blocking Details2% fat-free milk

Secondary Antibody

Secondary Descriptiongoat anti rat IgG, HRP

Details

Detection NotesECL, 10sec exposure, no primary antibody for negative controls

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FAQs for B220/CD45R Antibody (NB100-77420) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional B220/CD45R Products

B220/CD45R NB100-77420

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Recent Reviews

5
5
1
4
0
3
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2
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1
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Shijun Yan
06/29/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PTPRC
Entrez