Axl Antibody (MM0098-2N33) - Azide and BSA Free

Flow Cytometry: Axl Antibody (MM0098-2N33) [NBP2-12044] - A cell surface stain was performed on U87MG cells with Axl antibody (MM0098-2N33) NBP2-12044AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow (Cell Surface): Axl Antibody (MM0098-2N33) [NBP2-12044] - A cell surface stain was performed on HeLa cells with AXL antibody (MM0098-2N33) NBP2-12044PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: Axl Antibody (MM0098-2N33) [NBP2-12044] - Using the Alexa Fluor 647 direct conjugate A cell surface stain was performed on HeLa cells with AXL antibody (MM0098-2N33) NBP2-12044AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: Axl Antibody (MM0098-2N33) [NBP2-12044] - A cell surface stain was performed on HeLa cells with AXL antibody (MM0098-2N33) NBP2-12044AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu
• Applications: Flow, CyTOF-ready
• Clone: MM0098-2N33
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Axl Antibody (MM0098-2N33) - Azide and BSA Free Summary

• Immunogen: Human recombinant Axl (extracellular domain)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AXL
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1:50-100
• Application Notes: This antibody is Cytof ready.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31765380)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Axl Antibody (MM0098-2N33) - Azide and BSA Free

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl is encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axl Antibody (NBP2-12044)(2)

Publications using NBP2-12044

• Vogt MB, Lahon A, Arya RP, et al. Dengue viruses infect human megakaryocytes, with probable clinical consequences. PLoS Negl Trop Dis 2019-11-01 [PMID: 31765380] (FLOW, Mouse)
• Spencer J. L, Lahon A, et al. Replication of Zika Virus in Human Prostate Cells: A Potential Source of Sexually Transmitted Virus. J Infect Dis 2018-01-30 [PMID: 28968863] (FLOW, Human)

Bioinformatics

• Gene Symbol: AXL
• Uniprot:
  • AAA61243()