AVEN Antibody - BSA Free

Western Blot: AVEN Antibody [NBP1-76764] - Raji cell lysate with Aven antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: AVEN Antibody - BSA Free [NBP1-76764] - Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 u/mL.

Immunohistochemistry: AVEN Antibody - BSA Free [NBP1-76764] - Immunohistochemistry of AVEN in human spleen tissue with AVEN antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AVEN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AVEN Antibody - BSA Free (NBP1-76764) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Aven. The immunogen is located within the first 50 amino acids of Aven. Amino Acid Squence: QAERGARGGRGRRP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AVEN
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Despite its predicted molecular weight, Aven often migrates at 55 kDa in SDS-PAGE.
• Control Peptide:

  AVEN Peptide (NBP1-76764PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AVEN Antibody - BSA Free

• apoptosis, caspase activation inhibitor
• cell death regulator Aven
• PDCD12
• programmed cell death 12

Background

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9. It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients. Despite its predicted molecular weight, Aven often migrates at 55 kDa in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AVEN
• Uniprot:
  • NP_065104(Human)