Aurora B Antibody (6G2) - BSA Free

Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.

Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - Analysis of HeLa cells stained with mouse mAb to aurora B kinase, NBP2-50054, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. NBP2-50054 antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.

Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - HeLa cell cultures were stained with NBP2-50054 (green). Localization of Aurora B is cell cycle phase dependent. First, Aurora B staining was seen in chromosome arm in cells in prophase (green small dots). During anaphase, Aurora B localized in midzone and then concentrated in midbodies between the two daughter cells during telophase. It is therefore a useful marker of midbodies and dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.

• Reactivity: Hu, Ca, Eq
• Applications: WB, ICC/IF, IHC
• Clone: 6G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Aurora B Antibody (6G2) - BSA Free Summary

• Description: Novus Biologicals Mouse Aurora B Antibody (6G2) - BSA Free (NBP2-50054) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full length human Aurora B. [UniProt# Q96GD4]
• Specificity: This antibody does not cross-react with Aurora A or Aurora C.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AURKB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:2000
  • Immunohistochemistry 1:1000 - 1:2000
  • Western Blot 1:1000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aurora B Antibody (6G2) - BSA Free

• Aik2
• AIK2EC 2.7.11.1
• AIM1
• AIM-1
• AIM1Aik2
• AIM-1STK-1
• ARK2
• ARK2ARK-2
• AurB
• AURKB
• aurkb-sv2
• Aurora- and IPL1-like midbody-associated protein 1
• Aurora B
• aurora kinase BSerine/threonine-protein kinase aurora-B
• aurora kinase B-Sv1
• aurora kinase B-Sv2
• Aurora/IPL1-related kinase 2
• aurora-1
• aurora-B
• Aurora-related kinase 2
• EC 2.7.11
• IPL1
• serine/threonine kinase 12
• serine/threonine-protein kinase 12
• STK12
• STK12aurkb-sv1
• STK5

Background

Aurora-B is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process. Aurora-B is known to be involved in the regulation of centrosome function, bipolar spindle assembly and chromosome segregation. Aurora kinases are localized at the centrosomes of interphase cells, at the poles of the bipolar spindle and in the midbody of the mitotic apparatus. Substrates identified for the Aurora-B kinases include a kinesin-like motor protein, spindle apparatus proteins, histone H3 protein, kinetochore protein and the tumor suppressor protein p53 (1). Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. Identification of Aurora kinases as RasGAP Src homology 3 domain binding protein, also implicates these kinases as potential effectors in the Ras pathway relevant to oncogenesis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AURKB
• Uniprot:
  • Q96GD4()
  • Q96GD4()