ATR Antibody

Images

 
Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Validation of ATR antibody in Proximity Ligation Assay / PLA (which uses paired antibodies) and this image shows representative results for PLA using three color ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA n Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms), FITC/Green ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM6 (NB100-251) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM7 (NB100-252) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MSH6 (NB100-328) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. Goat anti-human RAD17 (NB100-272) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RENT1 (NB100-370) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPA2 (NBP2-22232) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA Hela cells. goat anti-human SA1 (NB100-298) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMC1 (NBP2-14858) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: ATR Antibody [NB100-323] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human SP1 (NBP2-22296) and rabbit anti-human ATR (NB100-323). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
 

Independent Antibodies

 

Biological Strategies

     

Order Details

ATR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human Ataxia Telangiectasia and Rad3-related using the numbering given in entry NP_001175.1 (GeneID 545).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:15000-1:30000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Theoretical MW
301 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-323 in the following applications:

  • WB
    2 publications

Reactivity Notes

The peptide used as the immunogen for this antibody shares 18 of 20 residues with Xenopus laevis. Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque, African elephant and Northern white-cheeked gibbon. Mouse reactivity reported in scientific literature (PMID:24356582).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATR Antibody

  • ataxia telangiectasia and Rad3 related
  • Ataxia telangiectasia and Rad3-related protein
  • ATR
  • EC 2.7.11.1
  • FRAP-related protein 1
  • FRP1
  • FRP1FRAP-related protein-1
  • MEC1
  • protein kinase ATR
  • Rad3 related protein
  • SCKL1
  • SCKLMEC1, mitosis entry checkpoint 1, homolog
  • serine/threonine-protein kinase ATR

Background

Ataxia and Rad3-related protein kinase (ATR) is a direct upstream activator of Chk1. This suggests that ATR plays a role in sensing DNA damage and initiating a subsequent protein kinase cascade.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NB100-464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
NB100-56546
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-384
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-143
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP, KO
NBP2-24457
Species: Hu, Mu, Pm
Applications: WB, IHC-P
NB100-598
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
NB100-142
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-22128
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-80695
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IF
NB120-13600
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-247
Species: Hu
Applications: WB, IHC-P, IP, PLA
NBP2-02004
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-304
Species: Hu, Mu, Rt, Fi, Gt, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NB100-56155
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-148
Species: Hu, Mu, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro
NBP1-85729
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-323
Species: Hu, Mu
Applications: WB, IP, PLA

Publications for ATR Antibody (NB100-323)(5)

Reviews for ATR Antibody (NB100-323) (0)

There are no reviews for ATR Antibody (NB100-323). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATR Antibody (NB100-323) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ATR Products

Bioinformatics Tool for ATR Antibody (NB100-323)

Discover related pathways, diseases and genes to ATR Antibody (NB100-323). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATR Antibody (NB100-323)

Discover more about diseases related to ATR Antibody (NB100-323).
 

Pathways for ATR Antibody (NB100-323)

View related products by pathway.

PTMs for ATR Antibody (NB100-323)

Learn more about PTMs related to ATR Antibody (NB100-323).
 

Research Areas for ATR Antibody (NB100-323)

Find related products by research area.

Blogs on ATR.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ATR Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ATR
Entrez
Uniprot