ATR Antibodies
We offer ATR Antibodies for use in common research applications: ELISA, Functional, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each ATR Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ATR Antibodies can be used in a variety of model species: Hamster, Human, Mouse, Rat. Use the list below to choose the ATR Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ATR polyclonal antibodies and browse our ATR monoclonal antibody catalog.
Alternate Names for ATR Antibodies
anti-ATR antibody, anti-ataxia telangiectasia and Rad3 related antibody, anti-Ataxia telangiectasia and Rad3-related protein antibody, anti-EC 2.7.11.1 antibody, anti-FRAP-related protein 1 antibody, anti-FRP1FRAP-related protein-1 antibody, anti-MEC1 antibody, anti-protein kinase ATR antibody, anti-Rad3 related protein antibody, anti-SCKL1 antibody, anti-SCKLMEC1, mitosis entry checkpoint 1, homolog antibody, anti-serine/threonine-protein kinase ATR antibody
14 Results for "atr" in Primary Antibodies
![Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0007.jpg "Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Immunocytochemistry-Immunofluorescence-NB100-308-img0001.jpg)
![Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0005.jpg)
![Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0004.jpg "Western Blot: ATR Antibody [NB100-323] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATR antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ATR Antibody [NB100-323] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ATR antibody NB100-323 lot 3 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ATR was also immunoprecipitated by a previous lot (lot 2) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0002.jpg)
![Western Blot: ATR Antibody [NB100-323] - H1299 (human lung cancer cells) and HCC 44 (human liver cancer cells). Antibody at 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ATR-Antibody-Western-Blot-NB100-323-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05447-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Rat testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0005.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Human testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0003.jpg)
![Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Mouse testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATR-[p-Ser428]-Antibody-Immunohistochemistry-Paraffin-NBP3-05447-img0004.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of 293T, using Phospho-ATR-S428 antibody at 1:400 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385445.jpg)
![Western Blot: ATR [p Ser428] Antibody [NBP3-05447] - Analysis of NIH/3T3, using Phospho-ATR-S428 antibody at 1:400 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37 for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-western-blot-265202385627.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Ser428] Antibody - Azide and BSA Free [ATR] - Immunofluorescence analysis of U2OS cells using ATR Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-05447_rabbit-polyclonal-atr-p-ser428-antibody-52202517202996.jpg)
![Western Blot: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Western-Blot-NBP3-13665-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunocytochemistry-Immunofluorescence-NBP3-13665-img0001.jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunohistochemistry-Paraffin-NBP3-13665-img0004.jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - ATR (phospho Thr1989) antibody [HL132] detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] (NBP3-13665) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-HL132-Immunohistochemistry-Paraffin-NBP3-13665-img0002.jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Rat lung.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13665_rabbit-atr-p-thr-1989-mab-hl132-immunohistochemistry-paraffin-910202391111..jpg)
![Immunohistochemistry-Paraffin: ATR [p Thr1989] Antibody (HL132) [NBP3-13665] - Mouse tissue.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody [HL132] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13665_rabbit-atr-p-thr-1989-mab-hl132-immunohistochemistry-paraffin-910202391240..jpg)
![Immunohistochemistry-Paraffin: ATR Antibody (1E9) [H00000545-M03] - Analysis of monoclonal antibody to ATR on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/ATR-Antibody-1E9-Immunohistochemistry-Paraffin-H00000545-M03-img0002.jpg "Immunohistochemistry-Paraffin: ATR Antibody (1E9) [H00000545-M03] - Analysis of monoclonal antibody to ATR on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml")
![Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [222]. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-222-Western-Blot-NBP2-43564-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody [222]. The signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Analysis of ATR protein from HCT-116 whole cell extracts using 5 ug of ATR antibody or ATR antibody. Western blot analysis was performed using ATR antibody. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/ATR-p-Thr1989-Antibody-222-Immunoprecipitation-NBP2-43564-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-910202419593539.jpg)
![Western Blot: ATR [p Thr1989] Antibody (222) [NBP2-43564] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [GT222] (NBP2-43564) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242014673.jpg)
![Immunocytochemistry/ Immunofluorescence: ATR [p Thr1989] Antibody (222) [NBP2-43564] - ATR antibody [GT222] detects ATR protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR stained by ATR antibody [GT222] (NBP2-43564) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43564_mouse-monoclonal-atr-p-thr1989-antibody-222-91020242075915.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-98982] - Immunofluorescence staining of ATR in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ATR polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/ATR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98982-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-98982] - Immunofluorescence staining of ATR in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ATR polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .")
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in mouse testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0004.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in human prostate with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0002.jpg)
![Immunohistochemistry-Paraffin: ATR Antibody [NBP2-98982] - Immunochemical staining ATR in human testis with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunohistochemistry-Paraffin-NBP2-98982-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATR Antibody (3F2) [H00000545-M01] - Analysis of monoclonal antibody to ATR on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ATR-Antibody-3F2-Immunocytochemistry-Immunofluorescence-H00000545-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: ATR Antibody (3F2) [H00000545-M01] - Analysis of monoclonal antibody to ATR on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: ATR Antibody (3F2) [H00000545-M01] - Detection limit for recombinant GST tagged ATR is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-3F2-ELISA-H00000545-M01-img0006.jpg)
![Western Blot: ATR Antibody (4O3E0) [NBP3-33305] - Western blot analysis of various lysates using ATR Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33305_rabbit-atr-mab-4o3e0-91220241418152.jpg "Western Blot: ATR Antibody (4O3E0) [NBP3-33305] - Western blot analysis of various lysates using ATR Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-76547] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76547-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATR Antibody [NBP2-76547] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.")
![Western Blot: ATR [p Ser428] Antibody (7F9W10) [NBP3-33572] - Western blot analysis of various lysates, using ATR Rabbit mAb at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33572_rabbit-atr-p-ser428-mab-7f9w10-101220241214417.jpg "Western Blot: ATR [p Ser428] Antibody (7F9W10) [NBP3-33572] - Western blot analysis of various lysates, using ATR Rabbit mAb at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")