ATP5D Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human lung cancer with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human hepatoma with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).

Western Blot: ATP5D Antibody [NBP3-00344] - NBP3-00344 at 1:500 dilution Lane A: HEPG2 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:14 kDa (We are unsure as to the identity of these extra bands.)

Immunoprecipitation: ATP5D Antibody [NBP3-00344] - ATP5D was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate Lane C:0.5 mg A549 Whole Cell Lysate 2 uL NBP3-00344 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00344, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 13 kDa Observed band size: 13 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATP5D Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human ATP5D (Uniprot#: P30049; Ala23-Glu168).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5F1D
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry 1:2500-1:10000
  • Immunohistochemistry-Paraffin 1:2500-1:10000
  • Immunoprecipitation 0.5-2 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ATP5D Antibody - Azide and BSA Free

• ATP synthase subunit delta, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
• F-ATPase delta subunit
• mitochondrial ATP synthase complex delta-subunit precusor
• mitochondrial ATP synthase, delta subunit

Background

The ATP5D gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATPsynthase is composed of two linke

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1D