Integrin alpha E/CD103 Antibody [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse Integrin alpha E/CD103 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1990) or (B) isotype control antibody (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha E/CD103 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103
  Phe20-Arg1113
  Accession # Q60677
• Specificity: Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGAE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha E/CD103 Antibody [Unconjugated]

• antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide
• CD103 antigen
• CD103
• HML-1 antigen
• HUMINAE
• Integrin alpha E
• integrin alpha-E
• Integrin alpha-IEL
• integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide)
• ITGAE
• MGC141996
• Mucosal lymphocyte 1 antigen

Background

The alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin  beta 7. Integrin  alpha E/ beta 7 is a receptor for E-Cadherin. Integrin  alpha E is expressed in intra‑epithelial lymphocytes and mediates adhesion of intra‑epithelial lymphocytes to epithelial cell monolayers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha E/CD103 Antibody (AF1990)(7)

Publications using AF1990

• Bosch, M;Kallin, N;Donakonda, S;Zhang, JD;Wintersteller, H;Hegenbarth, S;Heim, K;Ramirez, C;Fürst, A;Lattouf, EI;Feuerherd, M;Chattopadhyay, S;Kumpesa, N;Griesser, V;Hoflack, JC;Siebourg-Polster, J;Mogler, C;Swadling, L;Pallett, LJ;Meiser, P;Manske, K;de Almeida, GP;Kosinska, AD;Sandu, I;Schneider, A;Steinbacher, V;Teng, Y;Schnabel, J;Theis, F;Gehring, AJ;Boonstra, A;Janssen, HLA;Vandenbosch, M;Cuypers, E;Öllinger, R;Engleitner, T;Rad, R;Steiger, K;Oxenius, A;Lo, WL;Klepsch, V;Baier, G;Holzmann, B;Maini, MK;Heeren, R;Murray, PJ;Thimme, R;Herrmann, C;Protzer, U;Böttcher, JP;Zehn, D;Wohlleber, D;Lauer, GM;Hofmann, M;Luangsay, S;Knolle, PA; A liver immune rheostat regulates CD8 T cell immunity in chronic HBV infection Nature 2024-07-01 [PMID: 38987588] (Immunohistochemistry, Mouse, Transgenic Mouse)
• F Bayerl, DA Bejarano, G Bertacchi, AC Doffin, E Gobbini, M Hubert, L Li, P Meiser, AM Pedde, W Posch, L Rupp, A Schlitzer, M Schmitz, BU Schraml, S Uderhardt, J Valladeau-, D Wilflingse, V Zaderer, JP Böttcher Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods Oncogene, 2023-01-09;0(0):e2249923. 2023-01-09 [PMID: 36623939] (IHC, Mouse)
• Edurne Mugarza, Febe van Maldegem, Jesse Boumelha, Christopher Moore, Sareena Rana, Miriam Llorian Sopena et al. Therapeutic KRAS G12C inhibition drives effective interferon-mediated antitumor immunity in immunogenic lung cancers Science Advances 7/22/2022 [PMID: 35857848]
• Claudia A. Rivera, Violaine Randrian, Wilfrid Richer, Yohan Gerber-Ferder, Maria-Graciela Delgado, Aleksandra S. Chikina et al. Epithelial colonization by gut dendritic cells promotes their functional diversification Immunity 1/11/2022 [PMID: 34910930]
• Aleksandra S. Chikina, Francesca Nadalin, Mathieu Maurin, Mabel San-Roman, Thibault Thomas-Bonafos, Xin V. Li et al. Macrophages Maintain Epithelium Integrity by Limiting Fungal Product Absorption Cell 10/15/2020 [PMID: 32970988]
• JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control Cell, 2018-02-08;172(5):1022-1037.e14. 2018-02-08 [PMID: 29429633] (IHC-Fr, Mouse)
• SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017-02-20;0(0):. 2017-02-20 [PMID: 28219886] (Flow Cytometry, IHC, Mouse)

Bioinformatics

• Gene Symbol: ITGAE
• Uniprot:
  • Q60677()