| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92592-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00344-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86162-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47545-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92443-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97512-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB25031-SP | |||
![Western Blot: ATP5D Antibody [NBP2-92592] - Analysis of extracts of various cell lines, using ATP5D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Western-Blot-NBP2-92592-img0004.jpg "Western Blot: ATP5D Antibody [NBP2-92592] - Analysis of extracts of various cell lines, using ATP5D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: ATP5D Antibody [NBP2-92592] - Analysis of NIH/3T3 cells using ATP5D at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92592-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ATP5D Antibody [NBP2-92592] - Analysis of HeLa cells using ATP5D at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92592-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5D Antibody - BSA Free [NBP2-92592] - Immunofluorescence analysis of C6 cells using ATP5D antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92592_rabbit-polyclonal-atp5d-antibody-4220251426448.jpg%3Ft=1690983127)
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human lung cancer with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-23520251129340.png "Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human lung cancer with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP3-00344] - Immunochemical staining of human ATP5D in human hepatoma with NBP3-00344 (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-235202516144339.png)
![Western Blot: ATP5D Antibody [NBP3-00344] - NBP3-00344 at 1:500 dilution Lane A: HEPG2 Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:14 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-235202514492339.png)
![Immunoprecipitation: ATP5D Antibody [NBP3-00344] - ATP5D was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate Lane C:0.5 mg A549 Whole Cell Lysate 2 uL NBP3-00344 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00344, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 13 kDa Observed band size: 13 kDa](http://images.novusbio.com/fullsize/nbp3-00344_rabbit-polyclonal-atp5d-antibody-235202516335022.png)
![Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-86162] - Staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.](http://images.novusbio.com/fullsize/ATP5D-Antibody-Immunohistochemistry-Paraffin-NBP1-86162-img0001.jpg "Immunohistochemistry-Paraffin: ATP5D Antibody [NBP1-86162] - Staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.")
![Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-86162_rabbit-polyclonal-atp5d-antibody-24420259342994.jpg)
![Immunocytochemistry/Immunofluorescence: ATP5H Antibody [NBP2-47545] - Staining of human cell line U-251 MG shows localization to mitochondria.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47545-img0006.jpg "Immunocytochemistry/Immunofluorescence: ATP5H Antibody [NBP2-47545] - Staining of human cell line U-251 MG shows localization to mitochondria.")
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human liver.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-47545-img0010.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-47545-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human cerebral cortex, heart muscle, kidney and liver using Anti-ATP5H antibody NBP2-47545 (A) shows similar protein distribution across tissues to independent antibody NBP2-48906 (B).](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-47545-img0007.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human kidney.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-47545-img0008.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-47545-img0009.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-47545-img0011.jpg)
![Western Blot: ATP5H Antibody [NBP2-92443] - Analysis of extracts of various cell lines, using ATP5H at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Western-Blot-NBP2-92443-img0001.jpg "Western Blot: ATP5H Antibody [NBP2-92443] - Analysis of extracts of various cell lines, using ATP5H at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-92443] - Immunohistochemistry of paraffin-embedded mouse kidney using ATP5H Rabbit pAb (NBP2-92443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-92443-img0004.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-92443] - Immunohistochemistry of paraffin-embedded rat kidney using ATP5H Rabbit pAb (NBP2-92443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-92443-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5H Antibody - BSA Free [NBP2-92443] - Confocal immunofluorescence analysis of U2OS cells using ATP5H Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92443_rabbit-polyclonal-atp5h-antibody-4220251429055.jpg)
![Western Blot: ATP5H Antibody [NBP2-97512] - Anti-ATP5H rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: PC-3 Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/ATP5H-Antibody-Western-Blot-NBP2-97512-img0005.jpg "Western Blot: ATP5H Antibody [NBP2-97512] - Anti-ATP5H rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: PC-3 Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 20 kDa")
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-97512-img0004.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-97512-img0003.jpg)
![Immunoprecipitation: ATP5H Antibody [NBP2-97512] - ATP5H was immunoprecipitated using: Lane A: 0.5 mg PC-3 Whole Cell Lysate. 4 ul anti-ATP5H rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ATP5H rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunoprecipitation-NBP2-97512-img0006.jpg)
![Immunoprecipitation:ATP5H AntibodyNBP3-30298] -Detection of human ATP5H by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5H antibody NBP3-30298 (lot NBP3-30298-1) used for IP at 6 µg per reaction. ATP5H was also immunoprecipitated by rabbit anti-ATP5H.. For blotting immunoprecipitated ATP5H, NBP3-30298 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30298_rabbit-atp5h-pab-301220241316307.jpg "Immunoprecipitation:ATP5H AntibodyNBP3-30298] -Detection of human ATP5H by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP5H antibody NBP3-30298 (lot NBP3-30298-1) used for IP at 6 µg per reaction. ATP5H was also immunoprecipitated by rabbit anti-ATP5H.. For blotting immunoprecipitated ATP5H, NBP3-30298 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: ATP5H Antibody [NBP1-79479] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Western-Blot-NBP1-79479-img0003.jpg "Western Blot: ATP5H Antibody [NBP1-79479] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.")
![Western Blot: ATP5H Antibody [NBP1-79479] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Western-Blot-NBP1-79479-img0002.jpg)
![SDS-Page: ATP5H Protein [NBP2-22700]](http://images.novusbio.com/fullsize/ATP5H-Protein-SDS-Page-NBP2-22700-img0001.jpg "SDS-Page: ATP5H Protein [NBP2-22700]")
