ATM [p Ser1981] Antibody (10H11.E12)

Images

 
Immunohistochemistry: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - ATM [p Ser1981] antibody (10H11.E12) [NB100-306] was tested in mouse spleen using DAB with hematoxylin counterstain.
Western Blot: ATM [p Ser1981] Antibody (10H11.E12) [NB100-306] - Analysis of ATM-kinase, using ATM [p Ser1981] antibody (10H11.E12) [NB100-306]. Sample: Irradiated or peroxidated human fibroblasts. Observed molecular ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
10H11.E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ATM [p Ser1981] Antibody (10H11.E12) Summary

Immunogen
ATM [p Ser1981] Antibody (10H11.E12) was made to a synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]
Modification
p Ser1981
Localization
Nucleus, cytoplasm.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ATM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen.
Theoretical MW
351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-306 in the following applications:

  • 5 publications
  • IHC
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ATM [p Ser1981] Antibody (10H11.E12)

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutated
  • ATC
  • ATD
  • ATDC
  • ATE
  • ATM serine/threonine kinase
  • ATM
  • DKFZp781A0353
  • EC 2.7.11.1
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL

Background

ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.

References

1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATM Antibody (NB100-306)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(5)
IHC
(1)
WB
(7)
All Applications
Filter By Species
Human
(9)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-306 Applications Species
Gautam D, Stanley G, Owen M, Bridge E. Localization of the kinase Ataxia Telangiectasia Mutated to Adenovirus E4 mutant DNA replication centers is important for its inhibitory effect on viral DNA accumulation. Virology. Nov 16 2018 [PMID: 30453211] (ICC/IF, Human) ICC/IF Human
He DJ, Wang L, Zhang ZB et al. Maternal gene Ooep may participate in homologous recombination-mediated DNA double-strand break repair in mouse oocytes Zool Res Jun 15 2018 [PMID: 29955025] (Mouse) Mouse
Li Z, Liu J, Li J et al. Polo-like kinase 1 (Plk1) overexpression enhances ionizing radiation-induced cancer formation in mice J. Biol. Chem. 2017 Sep 12 [PMID: 28900036] (Mouse) Mouse
Wu PK, Wang JY, Chen CF et al. Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity. Stem Cells Transl Med Jun 1 2017 [PMID: 28544661] (WB, Human) WB Human
Liu X, Liu F, Gao S et al. A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5. Cancer Letters 2017 Jan 27 [PMID: 28137631] (ICC/IF, Human) ICC/IF Human
Banerjee K Banerjee S Mandal M. Enhanced chemotherapeutic efficacy of apigenin liposomes in colorectal cancer based on flavone-membrane interactions. J Colloid Interface Sci. Dec 18 2016 [PMID: 28012918] (IHC, Human) IHC Human
Takai H, Jenkinson E, Kabir S et al. A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus. Genes Dev. Apr 1 2016 [PMID: 27013236] (ICC/IF, Human) ICC/IF Human
Yang Z, Shen Y, Oishi H et al. Restoring oxidant signaling suppresses proarthritogenic T cell effector functions in rheumatoid arthritis. Sci Transl Med 2016 Mar 23 [PMID: 27009267] (WB, Human) WB Human
Caserta E, Egriboz O, Wang H et al. Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo. Genes Dev. 2015 Aug 15 [PMID: 26302789] (WB, Mouse) WB Mouse
LIEDTKE S, BIEBERNICK S, RADKE TF. DNA Damage Response in Neonatal and AdultStromal Cells Compared With Induced PluripotentStem Cells. Stem Cells Trans Med. 2015 Apr 21 [PMID: 25900727] (ICC/IF, Human) ICC/IF Human
Show All 22 Publications.

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Product General Protocols

View specific protocols for ATM Antibody (NB100-306): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATM Antibody (NB100-306). (Showing 1 - 6 of 6 FAQs).

  1. What is the theoretical molecular weight for your ATM antibodies?
    • The theoretical molecular weight for our ATM antibodies is 351 kDa.
  2. Is it possible to help me double confirm if these products contain any BSA? Catalog numbers: NB400-105, NB100-306, and NB100-64454.
    • No, none of these antibodies have BSA in their formulation.
  3. Is it possible to let me know what is the difference between NB100-307 and NB100-306 which are the same clone?
    • NB100-307 is unpurified mouse ascites, while NB100-306 is the purified version of the same antibody. They should detect similarly, with the purified one providing less background, and is tested in more applications.
  4. Do we have any information on possible cross reactivity of NB100-306 with 53BP1?
    • We do not currently have any testing data regarding the reactivity of our ATM (pSer1981) antibody with catalogue number NB100-306 against the 53BP1 protein. An alignment of the human protein sequences reveals an overall homology of just 20% however, so it is unlikely that NB100-306 would recognise 53BP1.
  5. Has NB100-306 been validated on IHC-Frozen?
    • No, we haven't tested this antibody in IHC-Fr application so far, but I do not see any reason on why it should not work in this application. Technically speaking, IHC-P protocols pose the target proteins/antigens to more harsh conditions/reagents as compared to IHC-Fr as well as ICC-IF procedures, and because NB100-306 worked very well in IHC-P and ICC-IF, we strongly believe that this product should work for IHC-Fr application also!
  6. What is the observed molecular weight for this antibody?
    • The observed molecular weight is 370 kDa.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-306AF350
Alexa Fluor 405 NB100-306AF405
Alexa Fluor 488 NB100-306AF488
Alexa Fluor 594 NB100-306AF594
Alexa Fluor 647 NB100-306AF647
Alexa Fluor 700 NB100-306AF700
Alexa Fluor 750 NB100-306AF750
Biotin NB100-306B
DyLight 350 NB100-306UV
DyLight 405 NB100-306V
DyLight 488 NB100-306G
DyLight 550 NB100-306R
DyLight 594 NB100-306DL594
DyLight 650 NB100-306C
DyLight 680 NB100-306FR
DyLight 755 NB100-306IR
FITC NB100-306F
HRP NB100-306H
Janelia Fluor 549 NB100-306JF549
Janelia Fluor 646 NB100-306JF646

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Blogs on ATM.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

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Bioinformatics

Gene Symbol ATM
Entrez
Uniprot