ATG9B Antibody

Images

 
Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: ATG9B Antibody [NBP1-77169] - HeLa cells with ATG9B antibody at 10 ug/mL.
Immunofluorescence: ATG9B Antibody [NBP1-77169] - Hela cells at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ATG9B Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B. The immunogen is located within amino acids 850 - 900 of ATG9B.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG9B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunofluorescence 20 ug/ml
Theoretical MW
101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ATG9B Peptide (NBP1-77169PEP)
Publications
Read Publication using
NBP1-77169 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATG9B Antibody

  • APG9L2APG9-like 2
  • ATG9 autophagy related 9 homolog B (S. cerevisiae)
  • autophagy-related protein 9B
  • endothelial nitric oxide synthase antisense
  • nitric oxide synthase 3 antisense
  • Nitric oxide synthase 3-overlapping antisense gene protein
  • NOS3ASautophagy 9-like 2 protein
  • Protein sONE
  • SONEFLJ14885

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IF

Publications for ATG9B Antibody (NBP1-77169)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG9B Antibody (NBP1-77169). (Showing 1 - 2 of 2 FAQ).

  1. How much is the molecular weight of ATG9B protein?
    • The predicted molecular weight of ATG9B protein is 101kDa
  2. Is this ATG9b antibody (NB110-74836) the same as the (discontinued?) R-144-100? Or are they different products?
    • Our product NB110-74836 is a different product than our discontinued product R-144-100. NB110-74836 is made against mouse autophagy-related protein 9B (ATG9B) and R-144-100 was made against human autophagy-related protein 9B (ATG9B). We have been recommending NBP1-77169 as a replacement product to R-144-100.

Secondary Antibodies

 

Isotype Controls

Additional ATG9B Products

ATG9B NBP1-77169

Bioinformatics Tool for ATG9B Antibody (NBP1-77169)

Discover related pathways, diseases and genes to ATG9B Antibody (NBP1-77169). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ATG9B Antibody (NBP1-77169)

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Pathways for ATG9B Antibody (NBP1-77169)

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Research Areas for ATG9B Antibody (NBP1-77169)

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Blogs on ATG9B.

TFEB - An essential regulator of lysosome biogenesis
Transcription factor EB (TFEB) is a member of the MiTF/TFE (Microphthalmia/TFE) subfamily of basic/helix-loop-helix/leucine zipper transcription factors. This group of proteins is involved in the proliferation and development of specific cell type...  Read full blog post.

Atg9b - a marker for autophagosome induction and assembly
Atg9 is the only essential transmembrane protein involved in cellular autophagy. Autophagy regulates cellular homeostasis by allowing the turnover and recycling of misfolded proteins and damaged organelles. Formation of the double-membrane isolatio...  Read full blog post.

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Bioinformatics

Gene Symbol ATG9B
Entrez
Uniprot