WIPI1 Antibody

Images

 
Orthogonal Strategies: Western Blot: WIPI1 Antibody [NBP1-88878] - Analysis in human cell lines SK-MEL-30 and HeLa using anti-WIPI1 antibody. Corresponding WIPI1 RNA-seq data are presented for the same cell ...read more
Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining in human placenta and fallopian tube tissues using anti-WIPI1 antibody. Corresponding WIPI1 RNA-seq data are presented for the same tissues.
Western Blot: WIPI1 Antibody [NBP1-88878] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Western Blot: WIPI1 Antibody [NBP1-88878] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human fallopian tube shows low expression as expected.
Immunohistochemistry-Paraffin: WIPI1 Antibody [NBP1-88878] - Staining of human placenta shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

WIPI1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HQDRAFATARLNFSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGECVLIKTHSLLGSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPIILCRG
Specificity
Specificity of human, mouse, rat WIPI1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WIPI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
WIPI1 Protein (NBP1-88878PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-88878 in the following applications:

Publications
Read Publication using
NBP1-88878 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26158518).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for WIPI1 Antibody

  • Atg18 protein homolog
  • Atg18
  • ATG18A
  • FLJ10055
  • WD repeat domain phosphoinositide-interacting protein 1
  • WD repeat domain, phosphoinositide interacting 1
  • WD40 repeat protein interacting with phosphoinositides of 49 kDa
  • WD40 repeat protein Interacting with phosphoInositides of 49kDa
  • WIPI 49 kDa
  • WIPI-1 alpha
  • WIPI-1
  • WIPI49ATG18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WIPI1 Antibody (NBP1-88878)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for WIPI1 Antibody (NBP1-88878) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-88878:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Simple Western WIPI1 NBP1-88878
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reviewed by:
Anonymous
Simple Western Mouse 03/25/2016
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Summary

ApplicationSimple Western
Sample Testedmouse whole brain lysate
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:1000, 1:250, 1:50

Details

Detection NotesWes Simple Western platform

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for WIPI1 Antibody (NBP1-88878) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for WIPI1 Antibody (NBP1-88878)

Discover related pathways, diseases and genes to WIPI1 Antibody (NBP1-88878). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for WIPI1 Antibody (NBP1-88878)

Discover more about diseases related to WIPI1 Antibody (NBP1-88878).
 

Pathways for WIPI1 Antibody (NBP1-88878)

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PTMs for WIPI1 Antibody (NBP1-88878)

Learn more about PTMs related to WIPI1 Antibody (NBP1-88878).

Blogs on WIPI1.

WIPI1 - An essential regulator of early autophagosome assembly
WD repeat domain phosphoinositide-interacting protein 1 (WIPI) is involved in the lysosomal degradation of cytoplasmic components during starvation-induced autophagy. WIPI1 is a seven bladed beta-propeller protein that provides a scaffold for the ...  Read full blog post.

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Recent Reviews

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Anonymous
03/25/2016
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol WIPI1