ATBF1/ZFHX3 Antibody [Unconjugated]

ATBF1/ZFHX3 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human ATBF1/ZFHX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7384) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ATBF1/ZFHX3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ATBF1/ZFHX3
  His3608-Leu3703
  Accession # Q15911
• Specificity: Detects human ATBF1/ZFHX3 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ZFHX3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ATBF1/ZFHX3 Antibody [Unconjugated]

• Alpha-fetoprotein enhancer-binding protein
• AT motif-binding factor 1
• AT motif-binding factor
• ATBF1
• ATBF1alpha-fetoprotein enhancer binding protein
• AT-binding transcription factor 1ATBT
• ATBT
• ZFH3
• ZFH-3
• ZFHX3
• zinc finger homeobox 3
• zinc finger homeobox protein 3
• Zinc finger homeodomain protein 3
• ZNF927

Background

ATBF1 (Adenine-Thymine motif Binding Factor 1; also ZFHX3) is a 400 kDa highly modular protein that contains motifs found in multiple transcription factor families. It is expressed by a variety of cells, both normal and malignant, including postmitotic neurons, hepatocytes, mammary epithelium and gastric carcinoma cells. ATBF1 is both cytoplasmic and nuclear, and its localization has pronounced effects on the cell cycle. Cytoplasmic ATBF1 is associated with cell proliferation, while nuclear ATBF1 appears to promote cell differentiation. Human ATBF1-A is 3703 amino acids (aa) in length. It contains seventeen consecutive C2H2-type Zn finger domains interspersed with short poly-Glu and poly-Ala segments (aa 282-2006), followed by two homeobox domains (aa 2146-2301), two additional C2H2 Zn finger domains (aa 2328-2552) and two homeobox plus three intermixed Zn finger domains. There are at least six utilized Ser phosphorylation sites. Two isoform variants have been reported. The first is termed ATBF1-B. It has a predicted MW of 306 kDa and appears following the use of an alternative start site at Met915. When the 914 aa N‑terminus is present (as in ATBF1-A), ATBF1 shows transcriptional repression; when it is absent (as in ATBF1-B), ATBF1 acts as a transcriptional activator. The second isoform variant contains a 41 aa substitution for aa 1150-3703. Over aa 3608-3703, human ATBF1 shares 98% aa sequence identity with mouse ATBF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATBF1/ZFHX3 Antibody (AF7384)(3)

Publications using AF7384

• Nishida, K;Matsumura, S;Uchida, H;Abe, M;Sakimura, K;Badea, TC;Kobayashi, T; Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons PloS one 2023-09-21 [PMID: 37733805] (IHC, Mouse)
• W Wang, H Cho, JW Lee, SK Lee The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development Nature Communications, 2022-02-17;13(1):958. 2/17/2022 [PMID: 35177643]
• Hayashi M, Hinckley CA, Driscoll SP, Moore NJ. Graded Arrays of Spinal and Supraspinal V2a Interneuron Subtypes Underlie Forelimb and Hindlimb Motor Control. Neuron. 2/21/2018 [PMID: 29398364]

Bioinformatics

• Gene Symbol: ZFHX3
• Uniprot:
  • Q15911(Human)