CCT3 Antibody

Western Blot: CCT3 Antibody [NBP1-79040] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79041 which recognizes a downstream epitope

Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79040] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).

Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79040] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).

• Reactivity: Hu, Mu, Rt, Pm-Or
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CCT3 Antibody Summary

• Description: Novus Biologicals Rabbit CCT3 Antibody (NBP1-79040) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human Chaperonin containing TCP1, Subunit 3 using the numbering given in entry NP_005989.3 (GeneID 7203).
• Predicted Species: Rat (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-10 ug/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCT3 Antibody

• Cctg
• chaperonin containing TCP1, subunit 3 (gamma)
• hTRiC5
• TCP1 (t-complex-1) ring complex, polypeptide 5
• TRIC5gamma subunit

Background

CCT3 encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCT3
• Uniprot:
  • NP_005989.3(Human)