ASGPR1 Antibody

Western Blot: ASGPR1 Antibody [NBP2-15489] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ASGPR1 antibody [N1C3] (NBP2-15489) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: ASGPR1 Antibody [NBP2-15489] - ASGPR1 antibody [N1C3] detects ASGPR1 protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ASGPR1 stained by ASGPR1 antibody [N1C3] (NBP2-15489) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10um.

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with ASGPR1 antibody [N1C3] (NBP2-15489) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ASGPR1 Antibody Summary

• Description: Novus Biologicals Rabbit ASGPR1 Antibody (NBP2-15489) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ASGPR1. The exact sequence is proprietary.
• Localization: Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASGR1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (80%), Pig (84%), Bovine (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ASGPR1 Antibody

• ASGPR 1
• ASGP-R 1
• ASGPR
• ASGPR1
• ASGR1
• asialoglycoprotein receptor 1
• CLEC4H1
• CLEC4H1ASGPR1
• C-type lectin domain family 4 member H1
• Hepatic lectin H1
• HL-1
• MHL1
• RHL1

Background

Partially deglycosylated plasma glycoproteins and immunoglobulin IgA2 allotypes are efficiently and specifically removed from circulation by a receptor-mediated process. The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate. Then the receptor is recycled back to the cell surface and the ligand is transported to the lysosomes for degradation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ASGR1
• Uniprot:
  • P07306()